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Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using ST6GALNAC6 Monoclonal antibody (66209-1-Ig)

    WB analysis of COLO 320 using 66209-1-Ig (same clone as 66209-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 66209-1-Ig (ST6GALNAC6 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66209-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using ST6GALNAC6 Monoclonal antibody (66209-1-Ig)

    IHC staining of mouse colon using 66209-1-Ig (same clone as 66209-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66209-1-Ig (ST6GALNAC6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66209-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using ST6GALNAC6 Monoclonal antibody (66209-1-Ig)

    IHC staining of mouse colon using 66209-1-Ig (same clone as 66209-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66209-1-Ig (ST6GALNAC6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66209-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using ST6GALNAC6 Monoclonal antibody (66209-1-Ig)

    IHC staining of human skin using 66209-1-Ig (same clone as 66209-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66209-1-Ig (ST6GALNAC6 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66209-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using ST6GALNAC6 Monoclonal antibody (66209-1-Ig)

    IHC staining of human skin using 66209-1-Ig (same clone as 66209-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66209-1-Ig (ST6GALNAC6 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66209-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using ST6GALNAC6 Monoclonal antibody (66209-1-Ig)

    IF Staining of human colon cancer using 66209-1-Ig (same clone as 66209-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using ST6GALNAC6 antibody (66209-1-Ig, Clone: 1A7B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66209-1-PBS in a different storage buffer formulation.

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ST6GALNAC6 Monoclonal antibody, PBS Only

ST6GALNAC6 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66209-1-PBS
Clone No.1A7B7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Synonyms

hST6GalNAc VI, RP11 203J24.3, Sialyltransferase 7F, SIAT7 F, SIAT7F, ST6GalNAc VI, ST6GALNAC6, ST6GALNACVI

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66209-1-PBS targets ST6GALNAC6 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ST6GALNAC6 fusion protein Ag8358 相同性解析による交差性が予測される生物種
Full Name ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6
Calculated molecular weight333aa,38 kDa; 374aa,41 kDa
Observed molecular weight 38-42 kDa
GenBank accession numberBC007802
Gene Symbol ST6GALNAC6
Gene ID (NCBI) 30815
RRIDAB_2881600
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ969X2
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ST6GALNAC6 is a sialyltransferase which is responsible for the synthesis of all a-series gangliosides. It has a broad expression pattern. ST6GALNAC6 was reported to be a key enzyme in the synthesis of disialyl monosialyl Lewis (Lea), a representative tumor-associated antigen in cancers of the pancreas and colon.