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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated SP1 Polyclonal antibody

SP1 Polyclonal Antibody for IF, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-21962

Synonyms

SP1, Sp1 transcription factor, Transcription factor Sp1, TSFP1

Validation Data Gallery

Applications:
  • IF Staining of HepG2 using CL488-21962

    IF Staining of HepG2 using CL488-21962

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 SP1 antibody (CL488-21962) at dilution of 1:800.

  • IF Staining of HepG2 using CL488-21962

    IF Staining of HepG2 using CL488-21962

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 SP1 antibody (CL488-21962) at dilution of 1:200.

  • FC experiment of HeLa using CL488-21962

    FC experiment of HeLa using CL488-21962

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SP1 (CL488-21962) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SP1 Ready-To-Use IHC Kit. SP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 21962-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL488-21962 is SP1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SP1 fusion protein Ag16720 相同性解析による交差性が予測される生物種
Full Name Sp1 transcription factor
Calculated molecular weight 785 aa, 81 kDa
Observed molecular weight 95-106 kDa
GenBank accession numberBC062539
Gene symbol SP1
Gene ID (NCBI) 6667
RRIDAB_2919186
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

The transcription factor Sp1 is a C2H2 zinc-finger protein that is involved in the regulation of a wide variety of genes, including housekeeping genes and tumor-developing genes. It is associated with tumor development, growth, and metastasis [PMID: 16209919]. It regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Besides, it has a role in modulating the cellular response to DNA damage, recruiting SMARCA4/BRG1 on the c-FOS promoter, regulation of FE65 gene expression [PMID:11371615,16332679]. SP1 is detected with MW 95-105 kDa and 65 kDa in this paper(PMID: 10618488).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 SP1 antibody CL488-21962Download protocol
FC protocol for CL Plus 488 SP1 antibody CL488-21962Download protocol
Standard Protocols
Click here to view our Standard Protocols