Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 21962-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21962-1-AP (SP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis using 21962-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21962-1-AP (SP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 21962-1-AP
WB result of SP1 antibody (21962-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SP1 transfected HeLa cells.
WB analysis of A431 using 21962-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 21962-1-AP (SP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 21962-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 21962-1-AP (SP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 21962-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 21962-1-AP (SP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 21962-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 21962-1-AP (SP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 21962-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 21962-1-AP (SP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 21962-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 21962-1-AP (SP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 21962-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 21962-1-AP (SP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of A431 using 21962-1-AP
IP result of anti-SP1 (IP:21962-1-AP, 6ug; Detection:21962-1-AP 1:500) with A431 cells lysate 2500ug.
IHC staining of human lung cancer using 21962-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21962-1-AP (SP1 Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human colon using 21962-1-AP
Immunohistochemical analysis of paraffin-embedded human colon using 21962-1-AP (SP1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon using 21962-1-AP
Immunohistochemical analysis of paraffin-embedded human colon using 21962-1-AP (SP1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human stomach cancer using 21962-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer using 21962-1-AP (SP1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human stomach cancer using 21962-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer using 21962-1-AP (SP1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lung cancer using 21962-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21962-1-AP (SP1 Antibody) at dilution of 1:200 (under 10x lens).
IF Staining of HeLa using 21962-1-AP
Immunofluorescent analysis of HeLa cells using 21962-1-AP (SP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

SP1 Polyclonal antibody

SP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 21962-1-AP

Publications(146)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Synonyms

SP1, Sp1 transcription factor, Transcription factor Sp1, TSFP1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (146)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Jurkat cells, A431 cells, HEK-293 cells, HeLa cells, K-562 cells
Positive IP detected in	A431 cells
Positive IHC detected in	human lung cancer tissue, human colon tissue, human stomach cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 23 publications below
WB	See 129 publications below
IHC	See 20 publications below
IF	See 20 publications below
IP	See 6 publications below
CoIP	See 4 publications below
ChIP	See 21 publications below

Product Information

21962-1-AP targets SP1 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity	human, mouse, rat, monkey
Cited Reactivity	human, mouse, rat, pig, monkey, sheep
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SP1 fusion protein Ag16720 相同性解析による交差性が予測される生物種
Full Name	Sp1 transcription factor
Calculated molecular weight	785 aa, 81 kDa
Observed molecular weight	95-106 kDa
GenBank accession number	BC062539
Gene Symbol	SP1
Gene ID (NCBI)	6667
ENSEMBL Gene ID	ENSG00000185591
RRID	AB_10898171
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P08047
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The transcription factor Sp1 is a C2H2 zinc-finger protein that is involved in the regulation of a wide variety of genes, including housekeeping genes and tumor-developing genes. It is associated with tumor development, growth, and metastasis [PMID: 16209919]. It regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Besides, it has a role in modulating the cellular response to DNA damage, recruiting SMARCA4/BRG1 on the c-FOS promoter, regulation of FE65 gene expression [PMID:11371615,16332679]. SP1 is detected with MW 95-105 kDa and 65 kDa in this paper(PMID: 10618488).

Protocols

Product Specific Protocols
WB protocol for SP1 antibody 21962-1-AP	Download protocol
IHC protocol for SP1 antibody 21962-1-AP	Download protocol
IF protocol for SP1 antibody 21962-1-AP	Download protocol
IP protocol for SP1 antibody 21962-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cancer Cell KMT2C deficiency drives transdifferentiation of double-negative prostate cancer and confer resistance to AR-targeted therapy Authors - Jiacheng Guo View Article
human	WB	Protein Cell Integrative analysis of transcriptome, DNA methylome and chromatin accessibility reveals candidate therapeutic targets in hypertrophic cardiomyopathy Authors - Junpeng Gao View Article
human	WB	Cell Res Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress. Authors - De Huang View Article
human	WB	Cell Rep Med Disruption of MerTK increases the efficacy of checkpoint inhibitor by enhancing ferroptosis and immune response in hepatocellular carcinoma Authors - Shun Wang View Article
mouse	WB	Sci Adv Targeting actin-bundling protein L-plastin as an anabolic therapy for bone loss. Authors - Xiaoqun Li View Article
human	WB	Nucleic Acids Res High mobility group AT-hook 1 (HMGA1) is an important positive regulator of hepatitis B virus (HBV) that is reciprocally upregulated by HBV X protein. Authors - Zhongliang Shen View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 21962-1-AP

WB analysis using 21962-1-AP

WB analysis of HeLa using 21962-1-AP

WB analysis of A431 using 21962-1-AP

WB analysis of HEK-293 using 21962-1-AP

WB analysis of Jurkat using 21962-1-AP

WB analysis of K-562 using 21962-1-AP

WB analysis of A431 using 21962-1-AP

WB analysis of Jurkat using 21962-1-AP

WB analysis of Jurkat using 21962-1-AP

IP experiment of A431 using 21962-1-AP

IHC staining of human lung cancer using 21962-1-AP

IHC staining of human colon using 21962-1-AP

IHC staining of human colon using 21962-1-AP

IHC staining of human stomach cancer using 21962-1-AP

IHC staining of human stomach cancer using 21962-1-AP

IHC staining of human lung cancer using 21962-1-AP

IF Staining of HeLa using 21962-1-AP

SP1 Polyclonal antibody

SP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

KMT2C deficiency drives transdifferentiation of double-negative prostate cancer and confer resistance to AR-targeted therapy

Integrative analysis of transcriptome, DNA methylome and chromatin accessibility reveals candidate therapeutic targets in hypertrophic cardiomyopathy

Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress.

Disruption of MerTK increases the efficacy of checkpoint inhibitor by enhancing ferroptosis and immune response in hepatocellular carcinoma

Targeting actin-bundling protein L-plastin as an anabolic therapy for bone loss.

High mobility group AT-hook 1 (HMGA1) is an important positive regulator of hepatitis B virus (HBV) that is reciprocally upregulated by HBV X protein.