Validation Data Gallery
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Tested Applications
| Positive FC (Intra) detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-82876 targets SAGE1 in FC (Intra) applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | SAGE1 fusion protein Ag34216 相同性解析による交差性が予測される生物種 |
| Full Name | sarcoma antigen 1 |
| Calculated molecular weight | 99kd |
| GenBank accession number | NM_018666.2 |
| Gene Symbol | SAGE1 |
| Gene ID (NCBI) | 55511 |
| RRID | AB_3673092 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9NXZ1 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Sarcoma antigen 1 (SAGE1) is a cancer/testis antigen that is limited to the spermatogonia and pre-meiotic spermatocytes and localized to the nuclei (PMID: 34465596).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 SAGE1 antibody CL488-82876 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |