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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MDA-MB-453 cells using SAGE1 Recombinant antibody (82876-1-RR)

    IF Staining of MDA-MB-453 using 82876-1-RR

    Immunofluorescent analysis of (4% PFA) fixed MDA-MB-453 cells using SAGE1 antibody (82876-1-RR, Clone: 230094C11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of U2OS cells using SAGE1 Recombinant antibody (82876-1-RR)

    FC experiment of U2OS using 82876-1-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug Anti-Human SAGE1 (82876-1-RR, Clone:230094C11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP)(blue) . Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 82876-1-RR

    Affinity and Kinetic Characterization of 82876-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82876-1-RR against Human SAGE1 were performed. The affinity constant is 0.546 nM.

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SAGE1 Recombinant antibody

SAGE1 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 82876-1-RR
Clone No.230094C11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

230094C11, Cancer/testis antigen 14, CT14, SAGE, sarcoma antigen 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inMDA-MB-453 cells
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82876-1-RR targets SAGE1 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SAGE1 fusion protein Ag34216 相同性解析による交差性が予測される生物種
Full Name sarcoma antigen 1
Calculated molecular weight99kd
GenBank accession numberNM_018666.2
Gene Symbol SAGE1
Gene ID (NCBI) 55511
RRIDAB_3670595
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NXZ1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sarcoma antigen 1 (SAGE1) is a cancer/testis antigen that is limited to the spermatogonia and pre-meiotic spermatocytes and localized to the nuclei (PMID: 34465596).

Protocols

Product Specific Protocols
IF protocol for SAGE1 antibody 82876-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols