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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MDA-MB-453 cells using SAGE1 Recombinant monoclonal antibody (82876-1-RR)

    IF Staining of MDA-MB-453 using 82876-1-RR (same clone as 82876-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MDA-MB-453 cells using SAGE1 antibody (82876-1-RR, Clone: 230094C11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82876-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U2OS cells using SAGE1 Recombinant monoclonal antibody (82876-1-RR)

    FC experiment of U2OS using 82876-1-RR (same clone as 82876-1-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug Anti-Human SAGE1 (82876-1-RR, Clone:230094C11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82876-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82876-1-RR

    Affinity and Kinetic Characterization of 82876-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82876-1-RR against Human SAGE1 were performed. The affinity constant is 0.546 nM.

SAGE1 Recombinant monoclonal antibody, PBS Only

SAGE1 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82876-1-PBS
Clone No.230094C11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Synonyms

230094C11, Cancer/testis antigen 14, CT14, SAGE, sarcoma antigen 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82876-1-PBS targets SAGE1 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag34216

Product name: Recombinant human SAGE1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 247-387 aa of NM_018666.2

Sequence: ATITYNVPEEKMEKGQPQPDNILSTASTGLINVAGAGTPAISTNGLYSTVPHNVCEEKMENDQPQPNNVLSTVQPVIIYLTATGIPGMNTRDQYATITHNVCEERVVNNQPLPSNALSTVLPGLAYLATADMPAMSTRDQH

相同性解析による交差性が予測される生物種
Full Name sarcoma antigen 1
Calculated molecular weight99kd
GenBank accession numberNM_018666.2
Gene Symbol SAGE1
Gene ID (NCBI) 55511
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9NXZ1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Sarcoma antigen 1 (SAGE1) is a cancer/testis antigen that is limited to the spermatogonia and pre-meiotic spermatocytes and localized to the nuclei (PMID: 34465596).

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