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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated S100A9 Polyclonal antibody (CL594-26992)

    IF Staining of HeLa using CL594-26992

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 S100A9 antibody (CL594-26992) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CoraLite®594-conjugated S100A9 Polyclonal antibody (CL594-26992)

    IF Staining of A549 using CL594-26992

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite®594 S100A9 antibody (CL594-26992) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CoraLite®594-conjugated S100A9 Polyclonal antibody (CL594-26992)

    IF Staining of U2OS using CL594-26992

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using CoraLite®594 S100A9 antibody (CL594-26992) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated S100A9 Polyclonal antibody (CL594-26992)

    FC experiment of HeLa using CL594-26992

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite®594 S100a9 Polyclonal Antibody (CL594-26992)(red), or 0.8 ug CoraLite®594-conjugated Rabbit IgG control Rabbit PolyAb (CL594-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated S100A9 Polyclonal antibody

S100A9 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-26992

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

CAGB, Calgranulin B, Calgranulin-B, Calprotectin L1H subunit, CFAG

Formulation:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells, A549 cells, U2OS cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-26992 targets S100A9 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag25764

Product name: Recombinant human S100A9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 25-114 aa of BC047681

Sequence: KLGHPDTLNQGEFKELVRKDLQNFLKKENKNEKVIEHIMEDLDTNADKQLSFEEFIMLMARLTWASHEKMHEGDEGPGHHHKPGLGEGTP

相同性解析による交差性が予測される生物種
Full Name S100 calcium binding protein A9
Calculated molecular weight 13 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC047681
Gene Symbol S100A9
Gene ID (NCBI) 6280
RRIDAB_3673429
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP06702
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

S100A9 is a calcium binding protein as a member of the S100 family of proteins. S100 proteins are low molecular weight (9 to 14 kDa) intracellular calcium-binding proteins that control key cellular pathways including regulation of the cytoskeleton, cell migration and adhesion, and host oxidative defense. S100A9 may exist as a homodimer, heterodimer with an S100A8 partner (S100A8/A9), or as a heterotetramer with an S100A8 partner(S100A8/A9). S100A8 and S100A9 are found intracellularly in granulocytes, monocytes, and early differentiation stages of macrophages.

Protocols

Product Specific Protocols
IF protocol for CL594 S100A9 antibody CL594-26992Download protocol
Standard Protocols
Click here to view our Standard Protocols
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