Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 26992-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 26992-1-AP (S100A9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 26992-1-AP
WB result of S100A9 antibody (26992-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-S100A9 transfected MCF-7 cells.
IP experiment of MCF-7 using 26992-1-AP
IP result of anti-S100A9 (IP:26992-1-AP, 4ug; Detection:26992-1-AP 1:3000) with MCF-7 cells lysate 1600 ug.
IHC staining of mouse spleen using 26992-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26992-1-AP (S100A9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 26992-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26992-1-AP (S100A9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 26992-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26992-1-AP (S100A9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 26992-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26992-1-AP (S100A9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse spleen using 26992-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26992-1-AP (S100A9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse spleen using 26992-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26992-1-AP (S100A9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 26992-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26992-1-AP (S100A9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 26992-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26992-1-AP (S100A9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human oesophagus cancer using 26992-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 26992-1-AP (S100A9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human oesophagus cancer using 26992-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 26992-1-AP (S100A9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 26992-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26992-1-AP (S100A9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 26992-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26992-1-AP (S100A9 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 26992-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using S100A9 antibody (26992-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of A549 using 26992-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using S100A9 antibody (26992-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 26992-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using S100A9 antibody (26992-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

S100A9 Polyclonal antibody

S100A9 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 26992-1-AP

Publications(66)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

L1AG, CFAG, Calprotectin L1H subunit, Calgranulin-B, Calgranulin B

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (66)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HL-60 cells, MCF-7 cells, THP-1 cells
Positive IP detected in	MCF-7 cells
Positive IHC detected in	human breast cancer tissue, human liver cancer tissue, mouse spleen tissue, human tonsillitis tissue, human oesophagus cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, A549 cells, HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 39 publications below
IHC	See 14 publications below
IF	See 18 publications below
IP	See 4 publications below
CoIP	See 2 publications below

Product Information

26992-1-AP targets S100A9 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat, sheep
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag25764 Product name: Recombinant human S100A9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 25-114 aa of BC047681 Sequence: KLGHPDTLNQGEFKELVRKDLQNFLKKENKNEKVIEHIMEDLDTNADKQLSFEEFIMLMARLTWASHEKMHEGDEGPGHHHKPGLGEGTP 相同性解析による交差性が予測される生物種
Full Name	S100 calcium binding protein A9
Calculated molecular weight	13 kDa
Observed molecular weight	14 kDa
GenBank accession number	BC047681
Gene Symbol	S100A9
Gene ID (NCBI)	6280
RRID	AB_2880716
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P06702
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

S100A9 is a calcium binding protein as a member of the S100 family of proteins. S100 proteins are low molecular weight (9 to 14 kDa) intracellular calcium-binding proteins that control key cellular pathways including regulation of the cytoskeleton, cell migration and adhesion, and host oxidative defense. S100A9 may exist as a homodimer, heterodimer with an S100A8 partner (S100A8/A9), or as a heterotetramer with an S100A8 partner(S100A8/A9). S100A8 and S100A9 are found intracellularly in granulocytes, monocytes, and early differentiation stages of macrophages.

Protocols

Product Specific Protocols
IF protocol for S100A9 antibody 26992-1-AP	Download protocol
IHC protocol for S100A9 antibody 26992-1-AP	Download protocol
IP protocol for S100A9 antibody 26992-1-AP	Download protocol
WB protocol for S100A9 antibody 26992-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Res Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2. Authors - Shaojun Zhang View Article
human	WB	Cell Rep Med Discovery of galectin-8 as an LILRB4 ligand driving M-MDSCs defines a class of antibodies to fight solid tumors Authors - Yiting Wang View Article
mouse		Sci Adv Gut microbiota from patients with arteriosclerotic CSVD induces higher IL-17A production in neutrophils via activating RORγt. Authors - Wei Cai View Article
human	CoIP	Theranostics Pancreatic ductal deletion of S100A9 alleviates acute pancreatitis by targeting VNN1-mediated ROS release to inhibit NLRP3 activation. Authors - Hong Xiang KD Validated View Article
mouse	WB,IHC	Theranostics Hepatic RACK1 deficiency protects against fulminant hepatitis through myeloid-derived suppressor cells. Authors - Genyu Liu View Article
mouse	WB	Phytomedicine Geniposide via enema alleviates colitis by modulating intestinal flora and bile acid metabolites, inhibiting S100A8/S100A9/NF-κB, and promoting TGR5 inhibition of NLRP3 inflammasome Authors - Bao-Xin Zheng View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 26992-1-AP

WB analysis of MCF-7 using 26992-1-AP

IP experiment of MCF-7 using 26992-1-AP

IHC staining of mouse spleen using 26992-1-AP

IHC staining of human breast cancer using 26992-1-AP

IHC staining of human breast cancer using 26992-1-AP

IHC staining of human liver cancer using 26992-1-AP

IHC staining of mouse spleen using 26992-1-AP

IHC staining of mouse spleen using 26992-1-AP

IHC staining of human tonsillitis using 26992-1-AP

IHC staining of human tonsillitis using 26992-1-AP

IHC staining of human oesophagus cancer using 26992-1-AP

IHC staining of human oesophagus cancer using 26992-1-AP

IHC staining of human tonsillitis using 26992-1-AP

IHC staining of human tonsillitis using 26992-1-AP

IF Staining of HeLa using 26992-1-AP

IF Staining of A549 using 26992-1-AP

IF Staining of HepG2 using 26992-1-AP

S100A9 Polyclonal antibody

S100A9 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2.

Discovery of galectin-8 as an LILRB4 ligand driving M-MDSCs defines a class of antibodies to fight solid tumors

Gut microbiota from patients with arteriosclerotic CSVD induces higher IL-17A production in neutrophils via activating RORγt.

Pancreatic ductal deletion of S100A9 alleviates acute pancreatitis by targeting VNN1-mediated ROS release to inhibit NLRP3 activation.

Hepatic RACK1 deficiency protects against fulminant hepatitis through myeloid-derived suppressor cells.

Geniposide via enema alleviates colitis by modulating intestinal flora and bile acid metabolites, inhibiting S100A8/S100A9/NF-κB, and promoting TGR5 inhibition of NLRP3 inflammasome