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Conjugate

ChromoTek RFP-Booster Alexa Fluor® 488

anti-RFP VHH/ Nanobody conjugated to fluorophore for IF, of mCherry and other red fluorescent protein-fusion proteins

Specificity

mCherry, mPlum, mRFP

Applications

IF, (FC, WB)

Conjugate

Alexa Fluor® 488

Host

Alpaca

Cat no : rb2AF488

Synonyms

RFP, RFP-Booster, Red fluorescent protein, mCherry, mRFP, mRFPRuby, mKate2 , mPlum, mOrange, mRuby2, mScarlet-I, PA-mCherry, TagRFP, RFP tag

Validation Data Gallery

  • RFP-Booster Alexa Fluor® 488 (rb2AF488, 1:500, green) immunostaining of HeLa cells transiently expressing mRFP-PCNA (red). Nuclei are stained with DAPI (blue). Confocal images were post-processed.

    RFP-Booster Alexa Fluor® 488 (rb2AF488, 1:500, green) immunostaining of HeLa cells transiently expressing mRFP-PCNA (red). Nuclei are stained with DAPI (blue). Confocal images were post-processed.

  • RFP-Booster Alexa Fluor® 488 (rb2AF488, 1:500, green) immunostaining of HeLa cells transiently expressing Vimentin-mCherry (red). Nucleoli are stained with anti-GNL3 (67169-1-Ig, 1:200, grey) pre-labeled with FlexAble 2.0 CoraLite® Plus 750 Antibody Labeling Kit for Mouse IgG2a (KFA544). Nuclei are stained with DAPI (blue). Confocal images were post-processed.

    RFP-Booster Alexa Fluor® 488 (rb2AF488, 1:500, green) immunostaining of HeLa cells transiently expressing Vimentin-mCherry (red). Nucleoli are stained with anti-GNL3 (67169-1-Ig, 1:200, grey) pre-labeled with FlexAble 2.0 CoraLite® Plus 750 Antibody Labeling Kit for Mouse IgG2a (KFA544). Nuclei are stained with DAPI (blue). Confocal images were post-processed.

Product Information

anti-RFP VHH/ Nanobody conjugated to fluorophore for IF, of mCherry and other red fluorescent protein-fusion proteins

DescriptionThe RFP-Booster stabilizes, enhances, and reactivates the signal of RFP-fusion proteins such as mCherry, mRFP, etc.. Due to its small size, the RFP-Booster enables higher image quality in epifluorescence, confocal, and super-resolution microscopy:

• Considerably higher tissue penetration rates

• Superior accessibility and labelling of epitopes in crowded cellular/organelle environments

• Less than 2 nm epitope-label displacement minimizes linkage error

• Monovalent VHHs do not cluster their epitopes

• Validation: structure and function characterized

• Consistent and reliable performance due to recombinant production

ApplicationsIF, (FC, WB)
HostAlpaca
Specificity/TargetmCherry, mPlum, mRFP, mRFPruby, mKate2
For the complete list, please click here: Fluorescent protein specificity table antibodies
ConjugateAlexa Fluor® 488
FormLiquid
Suggested Dilution /
TypeNanobody
FormatAlpaca single domain antibody, Nanobody or VHH; monovalent
ClassRecombinant
DOL2 fluorophores per Nanobody
RRID/
Affinity (KD)/
Excitation/ EmissionExcitation max: 490 nm, Emission max: 525 nm
Storage Buffer10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09% Sodium azide
Storage ConditionShipped at ambient temperature. Store at -20°C. Protect from light.
Size10 μL; 50 μL
NoteThis product is for research use only, not for diagnostic or therapeutic use