Validation Data Gallery
Product Information
anti-RFP VHH/ Nanobody conjugated to fluorophore for IF, of mCherry and other red fluorescent protein-fusion proteins
| Description | The RFP-Booster stabilizes, enhances, and reactivates the signal of RFP-fusion proteins such as mCherry, mRFP, etc.. Due to its small size, the RFP-Booster enables higher image quality in epifluorescence, confocal, and super-resolution microscopy: • Considerably higher tissue penetration rates • Superior accessibility and labelling of epitopes in crowded cellular/organelle environments • Less than 2 nm epitope-label displacement minimizes linkage error • Monovalent VHHs do not cluster their epitopes • Validation: structure and function characterized • Consistent and reliable performance due to recombinant production |
| Applications | IF, IHC, ICC |
| Host | Alpaca |
| Specificity/Target | mCherry, mPlum, mRFP, mRFPruby, mKate2 For the complete list, please click here: Fluorescent protein specificity table Nano-Booster |
| Conjugate | ATTO 647N |
| Physical State | Liquid |
| Suggested Dilution | IF/ICC/IHC: 1:200 |
| Type | Nanobody |
| Class | Recombinant |
| Format | Alpaca single domain antibody, Nanobody or VHH; monovalent |
| RRID | AB_2631391 |
| Affinity (KD) | Dissociation constant KD of 5nM |
| Excitation/ Emission | Excitation max: 647 nm, Emission max: 669 nm |
| Storage Buffer | 1x PBS, 0.09% sodium azide |
| Storage Condition | Shipped at ambient temperature. Store at -20°C/-4°F. Avoid freeze-thaw cycles. Aliquot upon arrival. Protect from light. Stable for 6 months. |
| Size | 10 μL; 100 μL |
| Note | This product is for research use only, not for diagnostic or therapeutic use |
Documentation
| SDS |
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| rba647n_SDS_RFP-Booster ATTO 647N (EN) |
| Datasheet |
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| Protocol RFP-Booster_ATTO647N (PDF) |
| Brochure |
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| Chromotek nanobodies brochure (PDF) |
| RFP-TRAP SPECIFICITY |
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| Fluorescent protein specificity table Nano-Booster |
Publications
| Application | Title |
|---|---|
Cell Deep Learning Reveals Cancer Metastasis and Therapeutic Antibody Targeting in the Entire Body. | |
Nat Cell Biol A role for Flower and cell death in controlling morphogen gradient scaling. | |
Neuron In Vivo Imaging of the Coupling between Neuronal and CREB Activity in the Mouse Brain. | |
Nat Commun Selective plasticity of callosal neurons in the adult contralesional cortex following murine traumatic brain injury. | |
Int J Mol Sci Visualization of Bacterial Protein Complexes Labeled with Fluorescent Proteins and Nanobody Binders for STED Microscopy |