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CoraLite® Plus 488-conjugated RCN2 Polyclonal antibody
RCN2 Polyclonal Antibody for IF
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF
Conjugate
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-10193
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive IF detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy RCN2 Ready-To-Use IHC Kit. RCN2 primary antibody included.
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL488-10193 targets RCN2 in IF applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RCN2 fusion protein Ag0247 相同性解析による交差性が予測される生物種 |
| Full Name | reticulocalbin 2, EF-hand calcium binding domain |
| Calculated molecular weight | 37 kDa |
| Observed molecular weight | 55 kDa |
| GenBank accession number | BC004892 |
| Gene symbol | RCN2 |
| Gene ID (NCBI) | 5955 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Reticulocalbin 2 (RCN2) is a Ca(2+)-binding protein that is specifically localized in the endoplasmic reticulum (ER). It is also termed ERC-55, i.e. ER calcium-binding protein of 55 kDa. It is a single copy gene and is encoded by an approximately 1900-base mRNA, which shows a ubiquitous expression pattern. The protein comprises an amino-terminal signal sequence followed by six copies of the EF-hand Ca2+ binding motif.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 RCN2 antibody CL488-10193 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
