Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, mouse lung tissue, human brain tissue, HeLa cells |
| Positive IP detected in | mouse lung tissue |
| Positive IHC detected in | human gliomas tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:350-1:1400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 10 publications below |
| IHC | See 6 publications below |
| IF | See 5 publications below |
| CoIP | See 1 publications below |
Product Information
10193-2-AP targets RCN2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0247 Product name: Recombinant human RCN2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 23-209 aa of BC004892 Sequence: GKAEELHYPLGERRSDYDREALLGVQEDVDEYVKLGHEEQQKRLQAIIKKIDLDSDGFLTESELSSWIQMSFKHYAMQEAKQQFVEYDKNSDDTVTWDEYNIQMYDRVIDFDENTALDDAEEESFRKLHLKDKKRFEKANQDSGPGLSLEEFIAFEHPEEVDYMTEFVIQEALEEHDKNGDGFVSLE 相同性解析による交差性が予測される生物種 |
| Full Name | reticulocalbin 2, EF-hand calcium binding domain |
| Calculated molecular weight | 37 kDa |
| Observed molecular weight | 55 kDa |
| GenBank accession number | BC004892 |
| Gene Symbol | RCN2 |
| Gene ID (NCBI) | 5955 |
| RRID | AB_2180018 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14257 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Reticulocalbin 2 (RCN2) is a Ca(2+)-binding protein that is specifically localized in the endoplasmic reticulum (ER). It is also termed ERC-55, i.e. ER calcium-binding protein of 55 kDa. It is a single copy gene and is encoded by an approximately 1900-base mRNA, which shows a ubiquitous expression pattern. The protein comprises an amino-terminal signal sequence followed by six copies of the EF-hand Ca2+ binding motif.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RCN2 antibody 10193-2-AP | Download protocol |
| IHC protocol for RCN2 antibody 10193-2-AP | Download protocol |
| IP protocol for RCN2 antibody 10193-2-AP | Download protocol |
| WB protocol for RCN2 antibody 10193-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Med Chemical corrector treatment ameliorates increased seizure susceptibility in a mouse model of familial epilepsy. | ||
Cell Metab A mechanosensitive lipolytic factor in the bone marrow promotes osteogenesis and lymphopoiesis. | ||
Proc Natl Acad Sci U S A Stk40 links the pluripotency factor Oct4 to the Erk/MAPK pathway and controls extraembryonic endoderm differentiation.
| ||
Elife Live-cell mapping of organelle-associated RNAs via proximity biotinylation combined with protein-RNA crosslinking. | ||
Elife Proteomic mapping of cytosol-facing outer mitochondrial and ER membranes in living human cells by proximity biotinylation. | ||
Am J Cancer Res YY1-mediated reticulocalbin-2 upregulation promotes the hepatocellular carcinoma progression via activating MYC signaling. |
