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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated RBM16 Polyclonal ant (CL488-18893)

    IF Staining of HeLa using CL488-18893

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 RBM16 antibody (CL488-18893) at dilution of 1:200, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated RBM16 Polyclonal ant (CL488-18893)

    FC experiment of HeLa using CL488-18893

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated RBM16 Polyclonal antibody (CL488-18893)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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CoraLite® Plus 488-conjugated RBM16 Polyclonal antibody

RBM16 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-18893

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Synonyms

RBM16, RNA binding protein 16, RNA-binding motif protein 16, SCAF8, SR-related and CTD-associated factor 8, CCAP7, CDC5L complex-associated protein 7, KIAA1116

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-18893 targets RBM16 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RBM16 fusion protein Ag13499 相同性解析による交差性が予測される生物種
Full Name RNA binding motif protein 16
Calculated molecular weight 1271 aa, 141 kDa
Observed molecular weight 170 kDa
GenBank accession numberBC070071
Gene Symbol RBM16
Gene ID (NCBI) 22828
RRIDAB_3672703
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UPN6
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RBM16, also named as SCAF8, CCAP7, KIAA1116, is a 1271 amino acid protein, which interacts with POLR2A. RBM16 may play a role in mRNA processing. Immunoblot analysis of the total rat liver nuclear and nuclear matrix protein fractions revealed a single band with an apparent molecular mass of 170 kDa. While the apparent molecular mass is slightly larger than the 140 kDa predicted from the cDNA sequence, we note that the protein is rich in proline residues (10%) and therefore may display aberrant mobility in SDS gel electrophoresis. (PMID: 9528809 )

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 RBM16 antibody CL488-18893Download protocol
FC protocol for CL Plus 488 RBM16 antibody CL488-18893Download protocol
Standard Protocols
Click here to view our Standard Protocols