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RBM16 Polyclonal antibody

RBM16 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 18893-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

SCAF8, RNA-binding motif protein 16, RNA binding protein 16, KIAA1116, CDC5L complex-associated protein 7

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inA549 cells, HeLa cells, NIH/3T3 cells, L02 cells, HT-1080 cells, THP-1 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman testis tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18893-1-AP targets RBM16 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RBM16 fusion protein Ag13499 相同性解析による交差性が予測される生物種
Full Name RNA binding motif protein 16
Calculated molecular weight 1271 aa, 141 kDa
Observed molecular weight 170 kDa
GenBank accession numberBC070071
Gene Symbol RBM16
Gene ID (NCBI) 22828
RRIDAB_2175908
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UPN6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RBM16, also named as SCAF8, CCAP7, KIAA1116, is a 1271 amino acid protein, which interacts with POLR2A. RBM16 may play a role in mRNA processing. Immunoblot analysis of the total rat liver nuclear and nuclear matrix protein fractions revealed a single band with an apparent molecular mass of 170 kDa. While the apparent molecular mass is slightly larger than the 140 kDa predicted from the cDNA sequence, we note that the protein is rich in proline residues (10%) and therefore may display aberrant mobility in SDS gel electrophoresis. (PMID: 9528809 )

Protocols

Product Specific Protocols
WB protocol for RBM16 antibody 18893-1-APDownload protocol
IHC protocol for RBM16 antibody 18893-1-APDownload protocol
IF protocol for RBM16 antibody 18893-1-APDownload protocol
IP protocol for RBM16 antibody 18893-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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