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Validation Data Gallery

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  • Flow cytometry (FC) experiment of HEK-293 cells using CoraLite® Plus 647-conjugated Phospho-c-SRC (Ser17 (CL647-80706-3)

    FC experiment of HEK-293 using CL647-80706-3

    1X10^6 HEK-293 cells were untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.25 ug Coralite® Plus 647 Phospho-C-SRC (Ser17) Recombinant Antibody (CL647-80706-3, Clone:250845F12)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

CoraLite® Plus 647-conjugated Phospho-c-SRC (Ser17) Recombinant monoclonal antibody

Phospho-c-SRC (Ser17) Uni-rAb® Recombinant Antibody for FC (Intra)
Cat No. CL647-80706-3
Clone No.250845F12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

c-SRC (Ser17), p-c-SRC, p-c-SRC (Ser17), p-c-SRC(Ser17), Phospho c-SRC

Formulation:  PBS, Proclin300, BSA, Glycerol
PBS, Proclin300, BSA, Glycerol
Conjugate:  CoraLite® Plus 647
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC (Intra) detected inλ phosphatase treated HEK-293 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-80706-3 targets Phospho-c-SRC (Ser17) in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
Calculated molecular weight 60 kDa
Observed molecular weight60 kDa
GenBank accession numberBC011566
Gene Symbol SRC
Gene ID (NCBI) 6714
ENSEMBL Gene IDENSG00000197122
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
激发激光Red Laser (633 nm)
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP12931
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. The phosphorylation of c-SRC at Ser17 is essential for its activation. This phosphorylation event is often mediated by protein kinase A (PKA) and is crucial for downstream signaling events. (PMID: 23360994)

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 Phospho-c-SRC (Ser17) antibody CL647-80706-3Download protocol
Standard Protocols
Click here to view our Standard Protocols
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