Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | HEK-293 cells, λ phosphatase treated HEK-293 cells |
| Positive IF/ICC detected in | HEK-293 cells |
| Positive FC (Intra) detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
80706-3-RR targets Phospho-c-SRC (Ser17) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) |
| Calculated molecular weight | 60 kDa |
| Observed molecular weight | 60 kDa |
| GenBank accession number | BC011566 |
| Gene Symbol | SRC |
| Gene ID (NCBI) | 6714 |
| ENSEMBL Gene ID | ENSG00000197122 |
| RRID | AB_3720379 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P12931 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. The phosphorylation of c-SRC at Ser17 is essential for its activation. This phosphorylation event is often mediated by protein kinase A (PKA) and is crucial for downstream signaling events. (PMID: 23360994)
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Phospho-c-SRC (Ser17) antibody 80706-3-RR | Download protocol |
| IF protocol for Phospho-c-SRC (Ser17) antibody 80706-3-RR | Download protocol |
| WB protocol for Phospho-c-SRC (Ser17) antibody 80706-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
iScience ESM1 drives cisplatin resistance and ferroptosis resistance via ERBB2/FAK/SRC/HSPB1/NF-κB signaling in ovarian cancer. | ||
Cell Death Discov MFAP2 secreted by TGF-β1-induced cancer-associated fibroblast cells promotes gastric cancer peritoneal metastasis through Src-STAT3-PTK7 axis. |