Validation Data Gallery
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Tested Applications
| Positive WB detected in | MG132 treated HT-1080 cells, MG132 treated NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85246-1-RR targets Phospho-Cyclin D1 (Thr286) in WB, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | cyclin D1 |
| Calculated molecular weight | 295 aa, 34 kDa |
| Observed molecular weight | 34 kDa |
| GenBank accession number | BC000076 |
| Gene Symbol | Cyclin D1 |
| Gene ID (NCBI) | 595 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P24385 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cyclin D1 is an important regulator of G1-S phase cell cycle transition. Active cyclin D1-cyclin dependent kinase 4/6 complexes phosphorylate retinoblastoma protein, resulting in release of sequestered E2F transcription factors and subsequent expression of genes required for progression into S phase. Cyclin D1 was localized to the nucleus and its rapid ubiquitin-dependent degradation shown to require phosphorylation at Thr286 by glycogen synthase kinase 3β (GSK3β). (PMID: 16504004)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-Cyclin D1 (Thr286) antibody 85246-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |