• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Phospho-Cyclin D1 (Thr286) Recombinant monoclonal (85246-1-RR)

    WB analysis using 85246-1-RR (same clone as 85246-1-PBS)

    Non-treated NIH/3T3 cells and MG132 treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 85246-1-RR (Cyclin D1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 85246-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-1080 cells using Phospho-Cyclin D1 (Thr286) Recombinant monoclonal (85246-1-RR)

    WB analysis of HT-1080 using 85246-1-RR (same clone as 85246-1-PBS)

    Non-treated HT-1080 cells and MG132 treated HT-1080 cells were subjected to SDS PAGE followed by western blot with 85246-1-RR (Phospho-Cyclin D1 (Thr286) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 85246-1-PBS in a different storage buffer formulation.

Phospho-Cyclin D1 (Thr286) Recombinant monoclonal antibody, PBS Only

Phospho-Cyclin D1 (Thr286) Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 85246-1-PBS
Clone No.242211A1

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, Indirect ELISA

Synonyms

Cyclin D1 (Thr286), p Cyclin D1, p Cyclin D1 (Thr286), p-Cyclin D1, p-Cyclin D1 (Thr286)

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85246-1-PBS targets Phospho-Cyclin D1 (Thr286) in WB, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name cyclin D1
Calculated molecular weight 295 aa, 34 kDa
Observed molecular weight34 kDa
GenBank accession numberBC000076
Gene Symbol Cyclin D1
Gene ID (NCBI) 595
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP24385
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Cyclin D1 is an important regulator of G1-S phase cell cycle transition. Active cyclin D1-cyclin dependent kinase 4/6 complexes phosphorylate retinoblastoma protein, resulting in release of sequestered E2F transcription factors and subsequent expression of genes required for progression into S phase. Cyclin D1 was localized to the nucleus and its rapid ubiquitin-dependent degradation shown to require phosphorylation at Thr286 by glycogen synthase kinase 3β (GSK3β). (PMID: 16504004)

||
New chat

Able™

正在加载，请稍候...