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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cyclophilin B Monoclonal antibody (66047-1-Ig)

    WB analysis using 66047-1-Ig

    HeLa/Jurkat/ROS1728/PC-12/C6 cells were subjected to SDS PAGE followed by western blot with 66047-1-Ig (Cyclophilin B antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Cyclophilin B Monoclonal antibody (66047-1-Ig)

    WB analysis of HeLa using 66047-1-Ig

    WB result of Cyclophilin B antibody (66047-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclophilin B transfected HeLa cells.

  • Western Blot (WB) analysis of HeLa cells using Cyclophilin B Monoclonal antibody (66047-1-Ig)

    WB analysis of HeLa using 66047-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66047-1-Ig (Cyclophilin B antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using Cyclophilin B Monoclonal antibody (66047-1-Ig)

    WB analysis of MCF-7 using 66047-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66047-1-Ig (Cyclophilin B antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using Cyclophilin B Monoclonal antibody (66047-1-Ig)

    WB analysis of HEK-293 using 66047-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66047-1-Ig (Cyclophilin B antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using Cyclophilin B Monoclonal antibody (66047-1-Ig)

    WB analysis of Jurkat using 66047-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66047-1-Ig (Cyclophilin B antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using Cyclophilin B Monoclonal antibody (66047-1-Ig)

    WB analysis of RAW 264.7 using 66047-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66047-1-Ig (Cyclophilin B antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Cyclophilin B Monoclonal antibody (66047-1-Ig)

    IHC staining of human pancreas cancer using 66047-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66047-1-Ig (Cyclophilin B antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Cyclophilin B Monoclonal antibody (66047-1-Ig)

    IHC staining of human pancreas cancer using 66047-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66047-1-Ig (Cyclophilin B antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Cyclophilin B Monoclonal antibody (66047-1-Ig)

    IHC staining of human pancreas cancer using 66047-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 66047-1-Ig(Cyclophilin B antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Cyclophilin B Monoclonal antibody (66047-1-Ig)

    IHC staining of human pancreas cancer using 66047-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 66047-1-Ig(Cyclophilin B antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Cyclophilin B Monoclonal antibody (66047-1-Ig)

    IF Staining of HeLa using 66047-1-Ig

    Immunofluorescent analysis of (4% PFA ) fixed HeLa cells using 66047-1-Ig(Cyclophilin B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Cyclophilin B Monoclonal antibody (66047-1-Ig)

    IF Staining of HeLa using 66047-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cyclophilin B antibody (66047-1-Ig, Clone: 8H4F1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using Cyclophilin B Monoclonal antibody (66047-1-Ig)

    FC experiment of HeLa using 66047-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Cyclophilin B (66047-1-Ig, Clone:8H4F1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Featured Product
  • KD/KO Validated

Cyclophilin B Monoclonal antibody

Cyclophilin B Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 66047-1-Ig
Clone No.8H4F1

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

PPIB, 8H4F1, CYP S1, CYPB, CYP-S1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, HEK-293 cells, Jurkat cells, RAW 264.7 cells, ROS1728 cells, PC-12 cells, C6 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66047-1-Ig targets Cyclophilin B in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag17957

Product name: Recombinant human PPIB protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-216 aa of BC032138

Sequence: MLRLSERNMKVLLAAALIAGSVFFLLLPGPSAADEKKKGPKVTVKVYFDLRIGDEDVGRVIFGLFGKTVPKTVDNFVALATGEKGFGYKNSKFHRVIKDFMIQGGDFTRGDGTGGKSIYGERFPDENFKLKHYGPGWVSMANAGKDTNGSQFFITTVKTAWLDGKHVVFGKVLEGMEVVRKVESTKTDSRDKPLKDVIIADCGKIEVEKPFAIAKE

相同性解析による交差性が予測される生物種
Full Name peptidylprolyl isomerase B (cyclophilin B)
Calculated molecular weight 216 aa, 24 kDa
Observed molecular weight 19-24 kDa
GenBank accession numberBC032138
Gene Symbol Cyclophilin B
Gene ID (NCBI) 5479
RRIDAB_11042327
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP23284
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PPIB, also named as CYPB, SCYLP, PPIase B and CYP-S1, belongs to the cyclophilin-type PPIase family and PPIase B subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIB is secreted in biological fluids such as blood or milk and binds to a specific receptor present on the human lymphoblastic cell line Jurkat and on human peripheral blood lymphocytes. The full-length of PPIB has 216aa (24 kDa), and a 33aa-signal peptide could be removed in the mature form (about 19 kDa).

Protocols

Product Specific Protocols
IF protocol for Cyclophilin B antibody 66047-1-IgDownload protocol
IHC protocol for Cyclophilin B antibody 66047-1-IgDownload protocol
WB protocol for Cyclophilin B antibody 66047-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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