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  • Featured Product
  • KD/KO Validated

Cyclophilin B Monoclonal antibody

Cyclophilin B Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

8H4F1

Cat no : 66047-1-Ig

Synonyms

Cyclophilin B, CYP S1, CYPB, PPIase B, PPIB, Rotamase B, S cyclophilin, SCYLP

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 66047-1-Ig

    WB analysis of HeLa using 66047-1-Ig

    WB result of Cyclophilin B antibody (66047-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclophilin B transfected HeLa cells.

  • WB analysis using 66047-1-Ig

    WB analysis using 66047-1-Ig

    HeLa/Jurkat/ROS1728/PC-12/C6 cells were subjected to SDS PAGE followed by western blot with 66047-1-Ig (Cyclophilin B antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of RAW 264.7 using 66047-1-Ig

    WB analysis of RAW 264.7 using 66047-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66047-1-Ig (Cyclophilin B antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 66047-1-Ig

    WB analysis of HeLa using 66047-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66047-1-Ig (Cyclophilin B antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 66047-1-Ig

    WB analysis of MCF-7 using 66047-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66047-1-Ig (Cyclophilin B antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 66047-1-Ig

    WB analysis of HEK-293 using 66047-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66047-1-Ig (Cyclophilin B antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 66047-1-Ig

    WB analysis of Jurkat using 66047-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66047-1-Ig (Cyclophilin B antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 66047-1-Ig

    IHC staining of human pancreas cancer using 66047-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66047-1-Ig (Cyclophilin B antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPIB antibody included
  • IHC staining of human pancreas cancer using 66047-1-Ig

    IHC staining of human pancreas cancer using 66047-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66047-1-Ig (Cyclophilin B antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPIB antibody included
  • IHC staining of human pancreas cancer using 66047-1-Ig

    IHC staining of human pancreas cancer using 66047-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 66047-1-Ig(Cyclophilin B antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with PPIB antibody included
  • IHC staining of human pancreas cancer using 66047-1-Ig

    IHC staining of human pancreas cancer using 66047-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 66047-1-Ig(Cyclophilin B antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with PPIB antibody included
  • IF Staining of HeLa using 66047-1-Ig

    IF Staining of HeLa using 66047-1-Ig

    Immunofluorescent analysis of (4% PFA ) fixed HeLa cells using 66047-1-Ig(Cyclophilin B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of HeLa using 66047-1-Ig

    IF Staining of HeLa using 66047-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cyclophilin B antibody (66047-1-Ig, Clone: 8H4F1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of HeLa using 66047-1-Ig

    FC experiment of HeLa using 66047-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Cyclophilin B (66047-1-Ig, Clone:8H4F1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, HEK-293 cells, Jurkat cells, RAW 264.7 cells, ROS1728 cells, PC-12 cells, C6 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PPIB Ready-To-Use IHC Kit. PPIB primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:400-1:1600
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

66047-1-Ig targets Cyclophilin B in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Cyclophilin B fusion protein Ag17957 相同性解析による交差性が予測される生物種
Full Name peptidylprolyl isomerase B (cyclophilin B)
Calculated molecular weight 216 aa, 24 kDa
Observed molecular weight 19-24 kDa
GenBank accession numberBC032138
Gene symbol PPIB
Gene ID (NCBI) 5479
RRIDAB_11042327
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PPIB, also named as CYPB, SCYLP, PPIase B and CYP-S1, belongs to the cyclophilin-type PPIase family and PPIase B subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIB is secreted in biological fluids such as blood or milk and binds to a specific receptor present on the human lymphoblastic cell line Jurkat and on human peripheral blood lymphocytes. The full-length of PPIB has 216aa (24 kDa), and a 33aa-signal peptide could be removed in the mature form (about 19 kDa).

Protocols

Product Specific Protocols
WB protocol for Cyclophilin B antibody 66047-1-IgDownload protocol
IHC protocol for Cyclophilin B antibody 66047-1-IgDownload protocol
IF protocol for Cyclophilin B antibody 66047-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Leon (Verified Customer) (07-06-2022)

Seems like a good antibody that detects cyclophilin B well and gave clear bands.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: SH-SY5Y
Cyclophilin B Antibody Western Blot validation (1:5000 dilution) in SH-SY5Y (Cat no:66047-1-Ig)