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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 488-conjugated Cyclophilin B Monocl (CL488-66047)

    WB analysis using CL488-66047

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66047 (Cyclophilin B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 488-conjugated Cyclophilin B Monocl (CL488-66047)

    WB analysis using CL488-66047

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66047 (Cyclophilin B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Cyclophilin B Monoclonal antibody

Cyclophilin B Monoclonal Antibody for WB
Cat No. CL488-66047
Clone No.8H4F1

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB

Synonyms

PPIB, Peptidyl-prolyl cis-trans isomerase B, EC:5.2.1.8, CYP-S1, CYPB

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66047 targets Cyclophilin B in WB applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Cyclophilin B fusion protein Ag17957 相同性解析による交差性が予測される生物種
Full Name peptidylprolyl isomerase B (cyclophilin B)
Calculated molecular weight 216 aa, 24 kDa
Observed molecular weight 19-24 kDa
GenBank accession numberBC032138
Gene Symbol Cyclophilin B
Gene ID (NCBI) 5479
RRIDAB_3084200
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP23284
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PPIB, also named as CYPB, SCYLP, PPIase B and CYP-S1, belongs to the cyclophilin-type PPIase family and PPIase B subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIB is secreted in biological fluids such as blood or milk and binds to a specific receptor present on the human lymphoblastic cell line Jurkat and on human peripheral blood lymphocytes. The full-length of PPIB has 216aa (24 kDa), and a 33aa-signal peptide could be removed in the mature form (about 19 kDa).

Protocols

Product Specific Protocols
WB protocol for CL Plus 488 Cyclophilin B antibody CL488-66047Download protocol
Standard Protocols
Click here to view our Standard Protocols