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  • Featured Product
  • KD/KO Validated

Cyclophilin B Monoclonal antibody, PBS Only

Cyclophilin B Monoclonal Antibody for WB, IF, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

8H4F1

Cat no : 66047-1-PBS

Synonyms

Cyclophilin B, CYP S1, CYPB, PPIase B, PPIB, Rotamase B, S cyclophilin, SCYLP

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 66047-1-Ig (same clone as 66047-1-PBS)

    WB analysis of HeLa using 66047-1-Ig (same clone as 66047-1-PBS)

    WB result of Cyclophilin B antibody (66047-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclophilin B transfected HeLa cells. This data was developed using the same antibody clone with 66047-1-PBS in a different storage buffer formulation.

  • WB analysis using 66047-1-Ig (same clone as 66047-1-PBS)

    WB analysis using 66047-1-Ig (same clone as 66047-1-PBS)

    HeLa/Jurkat/ROS1728/PC-12/C6 cells were subjected to SDS PAGE followed by western blot with 66047-1-Ig (Cyclophilin B antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66047-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 66047-1-Ig (same clone as 66047-1-PBS)

    WB analysis of Jurkat using 66047-1-Ig (same clone as 66047-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66047-1-Ig (Cyclophilin B antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66047-1-PBS in a different storage buffer formulation.

  • WB analysis of RAW 264.7 using 66047-1-Ig (same clone as 66047-1-PBS)

    WB analysis of RAW 264.7 using 66047-1-Ig (same clone as 66047-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66047-1-Ig (Cyclophilin B antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66047-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66047-1-Ig (same clone as 66047-1-PBS)

    WB analysis of HeLa using 66047-1-Ig (same clone as 66047-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66047-1-Ig (Cyclophilin B antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66047-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 66047-1-Ig (same clone as 66047-1-PBS)

    WB analysis of MCF-7 using 66047-1-Ig (same clone as 66047-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66047-1-Ig (Cyclophilin B antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66047-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66047-1-Ig (same clone as 66047-1-PBS)

    WB analysis of HEK-293 using 66047-1-Ig (same clone as 66047-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66047-1-Ig (Cyclophilin B antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66047-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 66047-1-Ig (same clone as 66047-1-PBS)

    IHC staining of human pancreas cancer using 66047-1-Ig (same clone as 66047-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66047-1-Ig (Cyclophilin B antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66047-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PPIB antibody included
  • IHC staining of human pancreas cancer using 66047-1-Ig (same clone as 66047-1-PBS)

    IHC staining of human pancreas cancer using 66047-1-Ig (same clone as 66047-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66047-1-Ig (Cyclophilin B antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66047-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PPIB antibody included
  • IHC staining of human pancreas cancer using 66047-1-Ig (same clone as 66047-1-PBS)

    IHC staining of human pancreas cancer using 66047-1-Ig (same clone as 66047-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 66047-1-Ig(Cyclophilin B antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66047-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PPIB antibody included
  • IHC staining of human pancreas cancer using 66047-1-Ig (same clone as 66047-1-PBS)

    IHC staining of human pancreas cancer using 66047-1-Ig (same clone as 66047-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 66047-1-Ig(Cyclophilin B antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66047-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PPIB antibody included
  • IF Staining of HeLa using 66047-1-Ig (same clone as 66047-1-PBS)

    IF Staining of HeLa using 66047-1-Ig (same clone as 66047-1-PBS)

    Immunofluorescent analysis of (4% PFA ) fixed HeLa cells using 66047-1-Ig(Cyclophilin B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66047-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66047-1-Ig (same clone as 66047-1-PBS)

    IF Staining of HeLa using 66047-1-Ig (same clone as 66047-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cyclophilin B antibody (66047-1-Ig, Clone: 8H4F1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66047-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 66047-1-Ig (same clone as 66047-1-PBS)

    FC experiment of HeLa using 66047-1-Ig (same clone as 66047-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Cyclophilin B (66047-1-Ig, Clone:8H4F1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66047-1-PBS in a different storage buffer formulation.

Tested Applications

Planning an IHC experiment? We recommend our IHCeasy PPIB Ready-To-Use IHC Kit. PPIB primary antibody included.

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

66047-1-PBS targets Cyclophilin B in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Cyclophilin B fusion protein Ag17957 相同性解析による交差性が予測される生物種
Full Name peptidylprolyl isomerase B (cyclophilin B)
Calculated molecular weight 216 aa, 24 kDa
Observed molecular weight 19-24 kDa
GenBank accession numberBC032138
Gene symbol PPIB
Gene ID (NCBI) 5479
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PPIB, also named as CYPB, SCYLP, PPIase B and CYP-S1, belongs to the cyclophilin-type PPIase family and PPIase B subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIB is secreted in biological fluids such as blood or milk and binds to a specific receptor present on the human lymphoblastic cell line Jurkat and on human peripheral blood lymphocytes. The full-length of PPIB has 216aa (24 kDa), and a 33aa-signal peptide could be removed in the mature form (about 19 kDa).