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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated PKIA Polyclonal antibody

PKIA Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11743

Synonyms

PKI alpha, PKIA, PRKACN1

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL488-11743

    IF Staining of HeLa using CL488-11743

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 PKIA antibody (CL488-11743) at dilution of 1:200.

Tested Applications

Positive IF detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 11743-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-11743 targets PKIA in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PKIA fusion protein Ag2338 相同性解析による交差性が予測される生物種
Full Name protein kinase (cAMP-dependent, catalytic) inhibitor alpha
Calculated molecular weight 76 aa, 8 kDa
GenBank accession numberBC022265
Gene symbol PKIA
Gene ID (NCBI) 5569
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen Affinity Purified
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

cAMP-dependent protein kinase inhibitor alpha (PKIA) is also named as PRKACN1, and belongs to the PKI family. Among them, PKIA, a member of protein kinase A (PKA) inhibitor family, was found to be most significantly and highly expressed in susceptible animals (PMID:24910983). Decreased PKIA signaling would directly impact PKA activation, potentially inducing DRP1 phosphorylation and increasing ER Ca2+ stores (PMID:32152556). miR-129-5p activated PKA to regulate the phosphorylation of beta-catenin and cAMP-response element binding protein (CREB) by inhibiting PKIA (PMID:36222334).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 PKIA antibody CL488-11743Download protocol
Standard Protocols
Click here to view our Standard Protocols