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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse embryo tissue using PKIA Polyclonal antibody (11743-1-AP)

    IHC staining of mouse embryo using 11743-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 11743-1-AP (PKIA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse embryo tissue using PKIA Polyclonal antibody (11743-1-AP)

    IHC staining of mouse embryo using 11743-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 11743-1-AP (PKIA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PKIA Polyclonal antibody (11743-1-AP)

    IHC staining of mouse brain using 11743-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11743-1-AP (PKIA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PKIA Polyclonal antibody (11743-1-AP)

    IHC staining of mouse brain using 11743-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11743-1-AP (PKIA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PKIA Polyclonal antibody (11743-1-AP)

    IF Staining of HeLa using 11743-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PKIA antibody (11743-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

PKIA Polyclonal antibody

PKIA Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 11743-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, ELISA

Synonyms

PRKACN1, PKI-alpha, PKI alpha, cAMP-dependent protein kinase inhibitor, muscle/brain isoform, cAMP-dependent protein kinase inhibitor alpha

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inmouse embryo tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11743-1-AP targets PKIA in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2338

Product name: Recombinant human PKIA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-76 aa of BC022265

Sequence: MTDVETTYADFIASGRTGRRNAIHDILVSSASGNSNELALKLAGLDINKTEGEEDAQRSSTEQSGEAQGEAAKSES

相同性解析による交差性が予測される生物種
Full Name protein kinase (cAMP-dependent, catalytic) inhibitor alpha
Calculated molecular weight 76 aa, 8 kDa
GenBank accession numberBC022265
Gene Symbol PKIA
Gene ID (NCBI) 5569
RRIDAB_3085380
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP61925
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

cAMP-dependent protein kinase inhibitor alpha (PKIA) is also named as PRKACN1, and belongs to the PKI family. Among them, PKIA, a member of protein kinase A (PKA) inhibitor family, was found to be most significantly and highly expressed in susceptible animals (PMID:24910983). Decreased PKIA signaling would directly impact PKA activation, potentially inducing DRP1 phosphorylation and increasing ER Ca2+ stores (PMID:32152556). miR-129-5p activated PKA to regulate the phosphorylation of beta-catenin and cAMP-response element binding protein (CREB) by inhibiting PKIA (PMID:36222334).

Protocols

Product Specific Protocols
IF protocol for PKIA antibody 11743-1-APDownload protocol
IHC protocol for PKIA antibody 11743-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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