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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated PIN1 Polyclonal antibody

PIN1 Polyclonal Antibody for IF, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10495

Synonyms

DOD, PIN1, PPIase Pin1, Rotamase Pin1, UBL5

Validation Data Gallery

Applications:
  • IF Staining of HEK-293 using CL488-10495

    IF Staining of HEK-293 using CL488-10495

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CoraLite® Plus 488 PIN1 antibody (CL488-10495) at dilution of 1:200.

  • FC experiment of HeLa using CL488-10495

    FC experiment of HeLa using CL488-10495

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human PIN1 (CL488-10495) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHEK-293 cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PIN1 Ready-To-Use IHC Kit. PIN1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10495-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-10495 targets PIN1 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PIN1 fusion protein Ag0767 相同性解析による交差性が予測される生物種
Full Name peptidylprolyl cis/trans isomerase, NIMA-interacting 1
Calculated molecular weight 18 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC002899
Gene symbol PIN1
Gene ID (NCBI) 5300
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

• PIN1(Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1) is essential for mitosis progression in yeast cells and is hypothesized to perform the same role in mammalian cells. It might regulate cellular processes distinct from the cell cycle itself, such as terminal differentiation through a modulation of differentiation-specific gene expression(PMID:20801874). It colocalizes with NEK6 in the nucleus. Pin1 inhibition simultaneously blocks multiple cancer pathways, disrupts the desmoplastic and immunosuppressive TME, and upregulates PD-L1 and ENT1, rendering pancreatic ductal adenocarcinoma (PDAC) eradicable by immunochemotherapy (PMID: 34388391).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 PIN1 antibody CL488-10495Download protocol
FC protocol for CL Plus 488 PIN1 antibody CL488-10495Download protocol
Standard Protocols
Click here to view our Standard Protocols