CoraLite®594-conjugated PHGDH Monoclonal antibody

PHGDH Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E8B8

Cat no : CL594-67591

Synonyms

3 PGDH, 3PGDH, PDG, PGAD, PGD, PGDH, PGDH3, PHGDH, phosphoglycerate dehydrogenase, SERA



Tested Applications

Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PHGDH Ready-To-Use IHC Kit. PHGDH primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67591-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-67591 targets PHGDH in IF applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PHGDH fusion protein Ag6877 相同性解析による交差性が予測される生物種
Full Name phosphoglycerate dehydrogenase
Calculated molecular weight 57 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC000303
Gene symbol PHGDH
Gene ID (NCBI) 26227
RRIDAB_2920161
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

PHGDH(D-3-phosphoglycerate dehydrogenase) is also named as 3-PGDH, PGDH3 and belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. It catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor. 3-PGDH deficiency is a rare recessive inborn error in the biosynthesis of the amino acid L-serine characterized clinically by congenital microcephaly, psychomotor retardation, and intractable seizures(PMID:19235232 ).

Protocols

Product Specific Protocols
IF protocol for CL594 PHGDH antibody CL594-67591Download protocol
Standard Protocols
Click here to view our Standard Protocols