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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated Profilin 1 Monoclonal antibody

Profilin 1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3A12E8

Cat no : CL594-67390

Synonyms

PFN1, profilin 1, Profilin I

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL594-67390

    IF Staining of HeLa using CL594-67390

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 Profilin 1 antibody (CL594-67390, Clone: 3A12E8 ) at dilution of 1:100.

Tested Applications

Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PFN1 Ready-To-Use IHC Kit. PFN1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67390-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-67390 targets Profilin 1 in IF applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Profilin 1 fusion protein Ag29206 相同性解析による交差性が予測される生物種
Full Name profilin 1
Calculated molecular weight 140 aa, 15 kDa
Observed molecular weight 13 kDa
GenBank accession numberBC006768
Gene symbol PFN1
Gene ID (NCBI) 5216
RRIDAB_2920125
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Profilin-1 (PFN1) plays an important role in the control of actin dynamics, and could represent an important therapeutic target in several diseases. PFN1 is identified as a huntingtin aggregation inhibitor, and may serves as a tumor-suppressor. PFN1 is crucial for the conversion of monomeric (G)-actin to filamentous (F)-actin. Amyotrophic lateral sclerosis (ALS) is a late-onset neurodegenerative disorder resulting from motor neuron death. Cells expressing PFN1 mutants contain ubiquitinated, insoluble aggregates that in many cases contain the ALS-associated protein TDP-43. Recently, PFN1 is a potential biomarker for bladder cancer aggressiveness and may be of great clinical importance.

Protocols

Product Specific Protocols
IF protocol for CL594 Profilin 1 antibody CL594-67390Download protocol
Standard Protocols
Click here to view our Standard Protocols