Validation Data Gallery
Product Information
PARP1-Trap Agarose is an affinity reagent for IP of human PARP1. It consists of a PARP1 Nanobody/ VHH, which is coupled to agarose beads.
| Description | Immunoprecipitation of PARP1 with PARP1-Trap Agarose.
• Effectively immunoprecipitates PARP1 (human) • Functional after IP: on-bead parylation assay • No heavy & light chains in your downstream application |
| Applications | IP, CoIP |
| Specificity/Target | PARP1, Epitope: Within DNA binding domain of PARP1 does not bind to PARP2, 3, and 9 |
| Tested Reactivity | Human |
| Conjugate | Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads) |
| Elution buffer | SDS sample buffer 0.2 M glycine pH 2.5 |
| Type | Nanobody |
| Class | Recombinant |
| Host | Alpaca |
| Compatibility with mass spectrometry | The PARP1-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry |
| RRID | AB_2631384 |
| Storage Buffer | 20% ethanol |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze! |
| Size | 25ul/reactions (eg：20rxns=500ul slurry) |
Publications
| Application | Title |
|---|---|
EMBO J Unanchored tri-NEDD8 inhibits PARP-1 to protect from oxidative stress-induced cell death. | |
EMBO Rep Ewing sarcoma protein promotes dissociation of poly(ADP-ribose) polymerase 1 from chromatin. | |
PLoS One A New Nanobody-Based Biosensor to Study Endogenous PARP1 In Vitro and in Live Human Cells. | |
Exp Mol Med Mitochondrial PARP1 regulates NAD(+)-dependent poly ADP-ribosylation of mitochondrial nucleoids |