Validation Data Gallery

  • Immunoprecipitation of PARP1 with PARP1-Trap.I: Input, FT: Flow-through, B: Bound.

    Immunoprecipitation of PARP1 with PARP1-Trap.I: Input, FT: Flow-through, B: Bound.

ChromoTek PARP1-Trap Agarose, Kit for Immunoprecipitation

PARP1-Trap Agarose is an affinity reagent for IP of human PARP1. It consists of a PARP1 Nanobody/ VHH, which is coupled to agarose beads.
Cat No. xtak

Specificity

PARP1

Reactivity

Human

Applications

IP, CoIP

Type

Nanobody

Conjugate

Agarose

PARP1-Trap, ADPRT, ADPRT 1, ADPRT1, pADPRT 1, PARP, PARP 1, PARP1, poly (ADP ribose) polymerase 1, Poly [ADP ribose] polymerase 1, Poly[ADP ribose] synthase 1, PPOL

Size/Concentration: 

-/ -


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Product Information

PARP1-Trap Agarose is an affinity reagent for IP of human PARP1. It consists of a PARP1 Nanobody/ VHH, which is coupled to agarose beads.

DescriptionThe PARP1-Trap Agarose Kit contains PARP1-Trap Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of p53.

• Effectively immunoprecipitates PARP1 (human)

• Functional after IP: on-bead parylation assay

• No heavy & light chains in your downstream application

ApplicationsIP, CoIP
Specificity/TargetPARP1, Epitope: Within DNA binding domain of PARP1
does not bind to PARP2, 3, and 9
Tested ReactivityHuman
ConjugateAgarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
TypeNanobody
ClassRecombinant
HostAlpaca
Compatibility with mass spectrometryThe PARP1-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2631384
Storage Buffer20% ethanol
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Kit components

ComponentDescription
PARP1-Trap Agarose beads20 reactions (500 µL)
Lysis bufferOptimized for cytoplasmatic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer Removal of unwanted proteins, peptides, etc.
Dilution bufferDilution of cell lysate
Elution bufferFor acidic elution