Validation Data Gallery
Product Information
PARP1-Trap Agarose is an affinity reagent for IP of human PARP1. It consists of a PARP1 Nanobody/ VHH, which is coupled to agarose beads.
| Description | The PARP1-Trap Agarose Kit contains PARP1-Trap Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of p53.
• Effectively immunoprecipitates PARP1 (human) • Functional after IP: on-bead parylation assay • No heavy & light chains in your downstream application |
| Applications | IP, CoIP |
| Specificity/Target | PARP1, Epitope: Within DNA binding domain of PARP1 does not bind to PARP2, 3, and 9 |
| Tested Reactivity | Human |
| Conjugate | Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads) |
| Elution buffer | SDS sample buffer 0.2 M glycine pH 2.5 |
| Type | Nanobody |
| Class | Recombinant |
| Host | Alpaca |
| Compatibility with mass spectrometry | The PARP1-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry |
| RRID | AB_2631384 |
| Storage Buffer | 20% ethanol |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze! |
Kit components
| Component | Description |
|---|---|
| PARP1-Trap Agarose beads | 20 reactions (500 µL) |
| Lysis buffer | Optimized for cytoplasmatic proteins and mammalian cell lysis |
| RIPA buffer | Optimized for nuclear/chromatin proteins and mammalian cell lysis |
| Wash buffer | Removal of unwanted proteins, peptides, etc. |
| Dilution buffer | Dilution of cell lysate |
| Elution buffer | For acidic elution |