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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated P15RS Polyclonal antibody

P15RS Polyclonal Antibody for IF, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-23652

Synonyms

HsT3101, p15INK4B related protein, P15RS, RPRD1A

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL488-23652

    IF Staining of HeLa using CL488-23652

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 P15RS antibody (CL488-23652) at dilution of 1:200, CL594-phalloidin (red).

  • IF Staining of HepG2 using CL488-23652

    IF Staining of HepG2 using CL488-23652

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 P15RS antibody (CL488-23652) at dilution of 1:200, CL594-phalloidin (red).

  • FC experiment of HeLa using CL488-23652

    FC experiment of HeLa using CL488-23652

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated P15RS Polyclonal antibody (CL488-23652)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHeLa cells, HepG2 cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 23652-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-23652 targets P15RS in IF, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen P15RS fusion protein Ag20409 相同性解析による交差性が予測される生物種
Full Name regulation of nuclear pre-mRNA domain containing 1A
Calculated molecular weight 312 aa, 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC010136
Gene symbol RPRD1A
Gene ID (NCBI) 55197
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RPRD1A is upregulated in cells overexpressing cyclin-dependent kinase inhibitor p15(INK4b) and may have a role in cell cycle regulation. It contains a RAR domain that is involved in regulation of nuclear pre-mRNA, which suggests that P15RS may act as a nuclear regulation protein [PMID:12470661]. It also interacts with phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD. Besides, RPRD1A regulates cell cycle genes and attenuates WNT signaling [PMID: 20739273], and acts as a negative regulator of cyclin-D1 (CCND1) and cyclin-E (CCNE1) in the cell cycle [PMID: 22231121].

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 P15RS antibody CL488-23652Download protocol
FC protocol for CL Plus 488 P15RS antibody CL488-23652Download protocol
Standard Protocols
Click here to view our Standard Protocols