Validation Data Gallery

Filter: AllWBIF/ICC

WB analysis using 23652-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 23652-1-AP (P15RS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 23652-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 23652-1-AP (P15RS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 23652-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 23652-1-AP (P15RS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 23652-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 23652-1-AP (P15RS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IF Staining of HeLa using 23652-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 23652-1-AP (P15RS antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 23652-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using P15RS antibody (23652-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
IF Staining of HepG2 using 23652-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using P15RS antibody (23652-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

Featured Product
KD/KO Validated

P15RS Polyclonal antibody

P15RS Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 23652-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

RPRD1A, Regulation of nuclear pre-mRNA domain-containing protein 1A, p15INK4B-related protein, p15INK4B related protein, HsT3101

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A431 cells, mouse liver tissue, mouse skeletal muscle tissue, L02 cells, Jurkat cells, HEK-293 cells
Positive IF/ICC detected in	HeLa cells, HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below
IHC	See 1 publications below
IF	See 1 publications below
IP	See 1 publications below

Product Information

23652-1-AP targets P15RS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag20409 Product name: Recombinant human P15RS protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 82-167 aa of BC010136 Sequence: APVIVEAFKHVSSETDESCKKHLGRVLSIWEERSVYENDVLEQLKQALYGDKKPRKRTYEQIKVDENENCSSLGSPSEPPQTLDLV 相同性解析による交差性が予測される生物種
Full Name	regulation of nuclear pre-mRNA domain containing 1A
Calculated molecular weight	312 aa, 36 kDa
Observed molecular weight	36 kDa
GenBank accession number	BC010136
Gene Symbol	RPRD1A
Gene ID (NCBI)	55197
RRID	AB_2879306
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96P16
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RPRD1A is upregulated in cells overexpressing cyclin-dependent kinase inhibitor p15(INK4b) and may have a role in cell cycle regulation. It contains a RAR domain that is involved in regulation of nuclear pre-mRNA, which suggests that P15RS may act as a nuclear regulation protein [PMID:12470661]. It also interacts with phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD. Besides, RPRD1A regulates cell cycle genes and attenuates WNT signaling [PMID: 20739273], and acts as a negative regulator of cyclin-D1 (CCND1) and cyclin-E (CCNE1) in the cell cycle [PMID: 22231121].

Protocols

Product Specific Protocols
WB protocol for P15RS antibody 23652-1-AP	Download protocol
IF protocol for P15RS antibody 23652-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Pharm Synergetic and Antagonistic Molecular Effects Mediated by the Feedback Loop of p53 and JNK between Saikosaponin D and SP600125 on Lung Cancer A549 Cells. Authors - Xiaoman Chen View Article
human	IP,WB,IHC,IF	Cell Death Dis RPRD1A stabilizes NRF2 and aggravates HCC progression through competing with p62 for TRIM21 binding. Authors - Xiaofan Feng KD Validated View Article
human	WB	Mol Cell Biol An Important Role for RPRD1B in the Heat Shock Response Authors - Simona Cugusi View Article
mouse	WB	Commun Biol Circ-0075305 hinders gastric cancer stem cells by indirectly disrupting TCF4-β-catenin complex and downregulation of SOX9 Authors - Qi-Yue Chen View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 23652-1-AP

WB analysis of mouse liver using 23652-1-AP

WB analysis of A431 using 23652-1-AP

WB analysis of mouse skeletal muscle using 23652-1-AP

IF Staining of HeLa using 23652-1-AP

IF Staining of HeLa using 23652-1-AP

IF Staining of HepG2 using 23652-1-AP