Validation Data Gallery
Product Information
Nano-Secondary® anti-rabbit IgG, recombinant VHH, is a rabbit IgG-specific antibody. This secondary antibody product consists of nanobodies that binds to the Fc-domain of rabbit IgG with high affinity & specificity.
| Description | Nano-Secondary® alpaca anti-rabbit IgG, recombinant VHH reagent uses a novel class of rabbit IgG-specific antibodies. This secondary antibody product consists of nanobodies that bind to rabbit IgG with high affinity & specificity. Due to their small size, Nano-Secondary reagents provide enhanced image resolution. No cross-reactivity against serum proteins from other species, see details below. |
| Applications | WB, IF |
| Host | Alpaca |
| Species Reactivity | Reactivity: Rabbit IgG No cross-reactivity: mouse, rat and human IgGs. |
| Target/Specificity | Rabbit IgG |
| Physical State | Liquid |
| Conjugate | CoraLite® Plus 750 |
| Suggested Dilution | Immunofluorescence 1:500 Western blot: 1:500 |
| Purification Method | Recombinant expression, affinity purification IMAC |
| Type | Secondary Nanobody |
| Class | Recombinant |
| Format | Alpaca single domain antibody, monovalent |
| Cross-reactivity | No cross-reactivity to mouse, rat or human IgGs |
| Immunogen | Purified rabbit IgG |
| Clonality | Biclonal: mixture of 2 monoclonal Nanobodies |
| Clones | CTK0119 (VHH0708), CTK0120 (VHH0953) |
| Affinity (KD) | CTK0119: KD = 0.2 nM, CTK120: KD = <10 pM |
| RRID | AB_3094642 |
| Excitation / Emission | Excitation max: 755 nm, Emission max: 780 nm |
| Storage Buffer | 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % Sodium azide |
| Storage Condition | Shipped at ambient temperature. Store at +4°C short term or -20°C long term. Stable for 1 year at -20°C. |
| Size | 10 μL; 100 μL |
| Note | This product is for research use only, not for diagnostic or therapeutic use |
Documentation
| SDS |
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| srb2GCL750-1_SDS_Nano-Secondary® alpaca anti-rabbit IgG, recombinant VHH, CoraLite® Plus 750 [CTK0119, CTK0120] (EN) |
| Datasheet |
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| Nano-Secondary® anti-rabbit IgG, recombinant VHH CoraLite® Plus 750 [CTK0119, CTK0120] Datasheet |
| Application note |
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| One-step Incubation with Nano-Secondaries (PDF) |
| Blocking recommendation for immunostaining with Nano-Secondaries (PDF) |
| Brochure |
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| Chromotek nanobodies brochure (PDF) |