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Validation Data Gallery

  • HEK-293 and Hela cell lysates were subjected to SDS-PAGE followed by fluorescent western blot analysis with rabbit anti-GAPDH antiboy (10494-1-AP) and Nano-Secondary® alpaca anti-rabbit IgG, recombinant VHH, CoraLite® Plus 488 (srb2GCL488-1), 1:500.

    HEK-293 and Hela cell lysates were subjected to SDS-PAGE followed by fluorescent western blot analysis with rabbit anti-GAPDH antiboy (10494-1-AP) and Nano-Secondary® alpaca anti-rabbit IgG, recombinant VHH, CoraLite® Plus 488 (srb2GCL488-1), 1:500.

  • Immunofluorescence analysis of A431 cells stained with rabbit anti-NCL antibody (10556-1-AP, green) and Nano-Secondary® alpaca anti-rabbit IgG, recombinant VHH, CoraLite® Plus 488 (srb2GCL488-1), 1:500. Nuclei were stained with DAPI (blue). Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

    Immunofluorescence analysis of A431 cells stained with rabbit anti-NCL antibody (10556-1-AP, green) and Nano-Secondary® alpaca anti-rabbit IgG, recombinant VHH, CoraLite® Plus 488 (srb2GCL488-1), 1:500. Nuclei were stained with DAPI (blue). Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

  • Immunofluorescence analysis of A431 cells co-stained with anti-EGFR antibody (cetuximab biosimilar, huIgG1, magenta) and anti-NCL antibody (10556-1-AP, green). Nano-Secondary® alpaca anti-human IgG, recombinant VHH, CoraLite® Plus 647 (shuGCL647-2), 1:500 and Nano-Secondary® alpaca anti-rabbit IgG, recombinant VHH, CoraLite® Plus 488 (srb2GCL488-1), 1:500 were used for detection. Nuclei were stained with DAPI. Merged image on the right shows an overlay of both channels and DAPI (blue). ​Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

    Immunofluorescence analysis of A431 cells co-stained with anti-EGFR antibody (cetuximab biosimilar, huIgG1, magenta) and anti-NCL antibody (10556-1-AP, green). Nano-Secondary® alpaca anti-human IgG, recombinant VHH, CoraLite® Plus 647 (shuGCL647-2), 1:500 and Nano-Secondary® alpaca anti-rabbit IgG, recombinant VHH, CoraLite® Plus 488 (srb2GCL488-1), 1:500 were used for detection. Nuclei were stained with DAPI. Merged image on the right shows an overlay of both channels and DAPI (blue). ​Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

ChromoTek Nano-Secondary® anti-rabbit IgG, recombinant VHH CoraLite® Plus 488 [CTK0119, CTK0120]

Nano-Secondary® anti-rabbit IgG, recombinant VHH, is a rabbit IgG-specific antibody. This secondary antibody product consists of nanobodies that binds to the Fc-domain of rabbit IgG with high affinity & specificity.
Cat No. srb2GCL488-1
Publications(2)

Applications

WB, IF

Conjugate

CoraLite® Plus 488

Host

Alpaca

Reactivity

Rabbit IgG

Synonyms

Alpaca single domain antibody, VHH, Nanobody, binding domain of single domain antibody, Nano-antibody

Formats:  CoraLite® Plus 488
Size: 

-/ -

Concentration: 0.5 mg/mL

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Product Information

Nano-Secondary® anti-rabbit IgG, recombinant VHH, is a rabbit IgG-specific antibody. This secondary antibody product consists of nanobodies that binds to the Fc-domain of rabbit IgG with high affinity & specificity.

DescriptionNano-Secondary® alpaca anti-rabbit IgG, recombinant VHH reagent uses a novel class of rabbit IgG-specific antibodies. This secondary antibody product consists of nanobodies that bind to rabbit IgG with high affinity & specificity. Due to their small size, Nano-Secondary reagents provide enhanced image resolution. No cross-reactivity against serum proteins from other species, see details below.
ApplicationsWB, IF
HostAlpaca
Species ReactivityReactivity: Rabbit IgG
No cross-reactivity: mouse, rat and human IgGs.
Target/SpecificityRabbit IgG
Physical StateLiquid
ConjugateCoraLite® Plus 488
Suggested DilutionImmunofluorescence 1:500
Western blot: 1:500
Purification MethodRecombinant expression, affinity purification IMAC
TypeSecondary Nanobody
ClassRecombinant
FormatAlpaca single domain antibody, monovalent
Cross-reactivityNo cross-reactivity to mouse, rat or human IgGs
ImmunogenPurified rabbit IgG
ClonalityBiclonal: mixture of 2 monoclonal Nanobodies
ClonesCTK0119 (VHH0708), CTK0120 (VHH0953)
Affinity (KD)CTK0119: KD = 0.2 nM, CTK120: KD = <10 pM
RRIDAB_3094639
Excitation / EmissionExcitation max: 490 nm, Emission max: 525 nm
Storage Buffer10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % Sodium azide
Storage ConditionShipped at ambient temperature. Store at +4°C short term or -20°C long term. Stable for 1 year at -20°C.
Size10 μL; 100 μL
NoteThis product is for research use only, not for diagnostic or therapeutic use

Publications

ApplicationTitle
IF

Front Cell Dev Biol

Screening for novel factors involved in mouse early embryonic development using inhibitor libraries.

Authors - Hirofumi Nishizono
View Article
IF

Front Pharmacol

Tanshinone IIA delays liver aging by modulating oxidative stress

Authors - Qi Liu
View Article
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