Validation Data Gallery

  • The alpaca anti-mouse IgG2a Fc-specific recombinant VHH [CTK0114]
has a very high affinity (KD <10 pM) for mouse IgG2a primary antibody.

    The alpaca anti-mouse IgG2a Fc-specific recombinant VHH [CTK0114] has a very high affinity (KD <10 pM) for mouse IgG2a primary antibody.

ChromoTek Nano-Secondary® alpaca anti-mouse IgG2a, recombinant VHH, for 2x Cys conjugation [CTK0114]

Monovalent, recombinant secondary single domain antibody to mouse IgG2a: alpaca monoclonal Nanobody, Fc-specific, for cysteine conjugation with thiol-reactive reagents, e.g. maleimides
Cat No. smsG2aCys2-1

Applications

IF, WB, FC

Reactivity

mouse IgG2a

Host

Alpaca

Conjugate

Unconjugated

Alpaca single domain antibody, VHH, Nanobody, binding domain of single domain antibody, Nano-antibody

Size/Concentration: 

-/ -


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Product Information

Monovalent, recombinant secondary single domain antibody to mouse IgG2a: alpaca monoclonal Nanobody, Fc-specific, for cysteine conjugation with thiol-reactive reagents, e.g. maleimides

ApplicationsIF, WB, FC
HostAlpaca-derived, recombinantly produced in bacteria
Species ReactivityMouse IgG2a Fc-fragment
No cross-reactivity: goat, guinea pig, rabbit, rat, sheep, human, macaque serum proteins, mouse IgG1, IgG2b, IgG2c, IgG3
Target/SpecificityFc-fragment of mouse IgG2a
Physical StateLiquid
ConjugateUnconjugated
Molecular weight14.4 kDa
Extinction coefficient (280 nm)24,200 M-1*cm-1
Purification MethodRecombinant expression, affinity purification IMAC
TypeSecondary Nanobody
ClassRecombinant
FormatAlpaca single domain antibody, monovalent
Cross-reactivityNo cross-reactivity to goat, guinea pig, human, macaque, rabbit, rat, and sheep serum and to mouse IgG1, IgG2b, IgG2c, and IgG3
ImmunogenPurified mouse IgG2a
ClonalityMonoclonal Nanobody
ClonesCTK0114
Affinity (KD)KD <10 pM
RRIDAB_2935700
Storage Buffer10 mM HEPES pH 7.0, 500 mM NaCl, 1 mM TCEP, Preservative: 0.09 % Sodium azide
Storage ConditionShipped on dry ice. Store at -80°C. Avoid freeze-thaw cycles. Stable for 1 year at -80°C.
Size500 µg
NoteThis product is for research use only, not for diagnostic or therapeutic use