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Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

  • Schematic representation of anti-mouse IgG2a Nano-Secondary binding to its target. Mouse IgG2a primary antibody (grey) is bound with two identical monoclonal mouse IgG2a Fc- specific Nanobody molecules (green). In total, 4 fluorophores (red stars) label the mouse IgG2a primary antibody.

    Schematic representation of anti-mouse IgG2a Nano-Secondary binding to its target. Mouse IgG2a primary antibody (grey) is bound with two identical monoclonal mouse IgG2a Fc- specific Nanobody molecules (green). In total, 4 fluorophores (red stars) label the mouse IgG2a primary antibody.

  • The alpaca anti-mouse IgG2a Fc-specific recombinant VHH [CTK0114] has a very high affinity (KD <10 pM) for mouse IgG2a primary antibody.

    The alpaca anti-mouse IgG2a Fc-specific recombinant VHH [CTK0114] has a very high affinity (KD <10 pM) for mouse IgG2a primary antibody.

  • Immunofluorescence analysis of Hela cells stained with mouse IgG2a anti-GLN3 antibody (67169-1-Ig) and Nano-Secondary® alpaca anti-mouse IgG2a, recombinant VHH, CoraLite® Plus 555 (magenta). Nuclei were stained with DAPI (blue). Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

    Immunofluorescence analysis of Hela cells stained with mouse IgG2a anti-GLN3 antibody (67169-1-Ig) and Nano-Secondary® alpaca anti-mouse IgG2a, recombinant VHH, CoraLite® Plus 555 (magenta). Nuclei were stained with DAPI (blue). Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

  • Flow cytometry analysis of 1X10^6 human peripheral blood mononuclear cells (PBMCs) stained with anti-human CD3 (clone OKT3, 65133-1-Ig) and Nano-Secondary® alpaca anti-mouse IgG2a, recombinant VHH, CoraLite® Plus 555.

    Flow cytometry analysis of 1X10^6 human peripheral blood mononuclear cells (PBMCs) stained with anti-human CD3 (clone OKT3, 65133-1-Ig) and Nano-Secondary® alpaca anti-mouse IgG2a, recombinant VHH, CoraLite® Plus 555.

ChromoTek Nano-Secondary® alpaca anti-mouse IgG2a, recombinant VHH, CoraLite® Plus 555 [CTK0114]

Nano-Secondary® alpaca anti-mouse IgG2a, Fc-specific recombinant VHH is an anti-mouse IgG subclass specific antibody. This secondary antibody product consists of Nanobodies that bind to mouse IgG2a with high affinity & specificity.
Cat No. smsG2aCL555-1

Applications

IF, FC

Reactivity

mouse IgG2a

Host

Alpaca

Conjugate

CoraLite® Plus 555

Synonyms

Alpaca single domain antibody, VHH, Nanobody, binding domain of single domain antibody, Nano-antibody

Size/Concentration: 

-/ -

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Product Information

Nano-Secondary® alpaca anti-mouse IgG2a, Fc-specific recombinant VHH is an anti-mouse IgG subclass specific antibody. This secondary antibody product consists of Nanobodies that bind to mouse IgG2a with high affinity & specificity.

DescriptionNano-Secondary® alpaca anti-mouse IgG2a, Fc-specific recombinant VHH reagent uses a novel class of anti-mouse IgG isotype specific antibodies. This secondary antibody product consists of Nanobodies that bind to mouse IgG2a with high affinity & specificity. Nano-Secondary reagents enable simultaneous incubation with primary antibodies, thus shortening your staining protocol. Due to their small size, Nano-Secondary reagents provide enhanced image resolution. No cross-reactivity against serum proteins from other species, details see below.
ApplicationsIF, FC
HostAlpaca-derived, recombinantly produced in bacteria
Species ReactivityMouse IgG2a Fc-fragment
No cross-reactivity: goat, guinea pig, rabbit, rat, sheep, human, macaque serum proteins, mouse IgG1, IgG2b, IgG2c, IgG3
Target/SpecificityFc-fragment of mouse IgG2a
Physical StateLiquid
ConjugateCoraLite® Plus 555
Suggested DilutionImmunofluorescence 1:500
Flow cytometry: 1µL per 1x106 cells.
Purification MethodRecombinant expression, affinity purification IMAC
TypeSecondary Nanobody
ClassRecombinant
FormatPurified human IgG
Cross-reactivityNo cross-reactivity to goat, guinea pig, human, macaque, rabbit, rat, and sheep serum and to mouse IgG1, IgG2b, IgG2c, and IgG3
ImmunogenPurified mouse IgG2a
ClonalityMonoclonal Nanobody
ClonesCTK0114 (VHH0913)
Affinity (KD)KD <10 pM
RRIDAB_2935697
Excitation / EmissionExcitation max: 554 nm, Emission max: 570 nm
Storage Buffer10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % Sodium azide
Storage ConditionShipped at ambient temperature. Store at +4°C short term or -20°C long term. Stable for 1 year at -20°C.
Size10 μL; 100 μL
NoteThis product is for research use only, not for diagnostic or therapeutic use