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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated NUDT21 Monoclonal antibody

NUDT21 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1C4C8

Cat no : CL594-66335

Synonyms

CFIM25, CPSF 25 kDa subunit, CPSF25, CPSF5, DKFZp686H1588, Nudix motif 21, NUDT21

Validation Data Gallery

Applications:
  • IF Staining of HepG2 using CL594-66335

    IF Staining of HepG2 using CL594-66335

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL594-66335 (NUDT21 antibody) at dilution of 1:100.

Tested Applications

Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy NUDT21 Ready-To-Use IHC Kit. NUDT21 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66335-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-66335 targets NUDT21 in IF applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NUDT21 fusion protein Ag12260 相同性解析による交差性が予測される生物種
Full Name nudix (nucleoside diphosphate linked moiety X)-type motif 21
Calculated molecular weight 26 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC001403
Gene symbol NUDT21
Gene ID (NCBI) 11051
RRIDAB_2883561
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cleavage and polyadenylation specific factor 5, 25 kDa (CPSF5, synonym: CFIM25) is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of CPSF5 with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors.

Protocols

Product Specific Protocols
IF protocol for CL594 NUDT21 antibody CL594-66335Download protocol
Standard Protocols
Click here to view our Standard Protocols