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  • Featured Product
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NUDT21 Monoclonal antibody

NUDT21 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IP, IF, RIP, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C4C8

Cat no : 66335-1-Ig

Synonyms

CFIM25, CPSF 25 kDa subunit, CPSF25, CPSF5, Nudix motif 21, NUDT21

Validation Data Gallery

Applications:
  • WB analysis of NIH/3T3 using 66335-1-Ig

    WB analysis of NIH/3T3 using 66335-1-Ig

    WB result of NUDT21 antibody (66335-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NUDT21 transfected NIH/3T3 cells.

  • WB analysis of NIH/3T3 using 66335-1-Ig

    WB analysis of NIH/3T3 using 66335-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66335-1-Ig (NUDT21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 66335-1-Ig

    WB analysis of HEK-293 using 66335-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66335-1-Ig (NUDT21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 66335-1-Ig

    WB analysis of HeLa using 66335-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66335-1-Ig (NUDT21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 66335-1-Ig

    WB analysis of MCF-7 using 66335-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66335-1-Ig (NUDT21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 66335-1-Ig

    IHC staining of mouse brain using 66335-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NUDT21 antibody included
  • IHC staining of human brain using 66335-1-Ig

    IHC staining of human brain using 66335-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NUDT21 antibody included
  • IHC staining of human brain using 66335-1-Ig

    IHC staining of human brain using 66335-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NUDT21 antibody included
  • IHC staining of human brain using 66335-1-Ig

    IHC staining of human brain using 66335-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NUDT21 antibody included
  • IHC staining of mouse brain using 66335-1-Ig

    IHC staining of mouse brain using 66335-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NUDT21 antibody included
  • IHC staining of mouse brain using 66335-1-Ig

    IHC staining of mouse brain using 66335-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NUDT21 antibody included
  • IHC staining of mouse liver using 66335-1-Ig

    IHC staining of mouse liver using 66335-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NUDT21 antibody included
  • IHC staining of mouse liver using 66335-1-Ig

    IHC staining of mouse liver using 66335-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NUDT21 antibody included
  • IHC staining of human brain using 66335-1-Ig

    IHC staining of human brain using 66335-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NUDT21 antibody included
  • IF Staining of HeLa using 66335-1-Ig

    IF Staining of HeLa using 66335-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NUDT21 antibody (66335-1-Ig, Clone: 1C4C8 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

  • IF Staining of HepG2 using 66335-1-Ig

    IF Staining of HepG2 using 66335-1-Ig

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66335-1-Ig(NUDT21 antibody) at dilution of 1:400 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of HepG2 using 66335-1-Ig

    IF Staining of HepG2 using 66335-1-Ig

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66335-1-Ig(NUDT21 antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inNIH/3T3 cells, HEK-293 cells, HeLa cells, MCF-7 cells
Positive IHC detected inhuman brain tissue, mouse brain tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy NUDT21 Ready-To-Use IHC Kit. NUDT21 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

66335-1-Ig targets NUDT21 in WB, IP, IF, RIP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NUDT21 fusion protein Ag12260 相同性解析による交差性が予測される生物種
Full Name nudix (nucleoside diphosphate linked moiety X)-type motif 21
Calculated molecular weight 26 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC001403
Gene symbol NUDT21
Gene ID (NCBI) 11051
RRIDAB_2881715
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cleavage and polyadenylation specific factor 5, 25 kDa (CPSF5, synonym: CFIM25) is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of CPSF5 with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors.

Protocols

Product Specific Protocols
WB protocol for NUDT21 antibody 66335-1-IgDownload protocol
IHC protocol for NUDT21 antibody 66335-1-IgDownload protocol
IF protocol for NUDT21 antibody 66335-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIP

Mol Cell

A Cancer-Specific Ubiquitin Ligase Drives mRNA Alternative Polyadenylation by Ubiquitinating the mRNA 3' End Processing Complex.

Authors - Seung Wook Yang
View Article
humanWB,IF,IP,RIP

Cell Rep

LINC00921 reduces lung cancer radiosensitivity by destabilizing NUDT21 and driving aberrant MED23 alternative polyadenylation

Authors - Nasha Zhang
View Article
humanWB

J Mol Cell Biol

U1 snRNP proteins promote proximal alternative polyadenylation sites by directly interacting with 3' end processing core factors

Authors - Zhijie Hu
View Article