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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated NCL Polyclonal antibody

NCL Polyclonal Antibody for IF, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10556

Synonyms

C23, FLJ45706, NCL, nucleolin, Protein C23

Validation Data Gallery

Applications:
  • IF Staining of HEK-293 using CL488-10556

    IF Staining of HEK-293 using CL488-10556

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CoraLite® Plus 488 NCL antibody (CL488-10556) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HeLa using CL488-10556

    FC experiment of HeLa using CL488-10556

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human NCL (CL488-10556) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF detected inHEK-293 cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NCL Ready-To-Use IHC Kit. NCL primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10556-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-10556 targets NCL in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NCL fusion protein Ag0859 相同性解析による交差性が予測される生物種
Full Name nucleolin
Calculated molecular weight 76 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC006494
Gene symbol NCL
Gene ID (NCBI) 4691
RRIDAB_2918986
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nucleolin, also known as C23, involved in the control of transcription of ribosomal RNA (rRNA) genes by RNA polymerase I, in nucleocytoplasmic transportation of ribosomal components, and in ribosome maturation and assembly. It associated with intranucleolar chromatin and pre-ribosomal particles, and induced chromatin decondensation by binding to histione H1. Also it has a role in the process of transcriptional elongation. Whilst mammalian nucleolin has a predicted molecular mass of approximately 77 kDa, the apparent molecular mass is between 100 and 110 kDa, and has been attributed to the amino acid composition of the N-terminal domain, which is highly phosphorylated. (PMID: 15925566)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 NCL antibody CL488-10556Download protocol
FC protocol for CL Plus 488 NCL antibody CL488-10556Download protocol
Standard Protocols
Click here to view our Standard Protocols