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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite® Plus 488-conjugated MYL9 Polyclonal anti (CL488-15354)

    IF Staining of MCF-7 using CL488-15354

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 MYL9 antibody (CL488-15354) at dilution of 1:100.

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  • KD/KO Validated

CoraLite® Plus 488-conjugated MYL9 Polyclonal antibody

MYL9 Polyclonal Antibody for IF/ICC
Cat No. CL488-15354

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

20 kDa myosin light chain, LC20, MLC 2C, MLC2, MRLC1, MYL9, Myosin RLC, MYRL2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-15354 targets MYL9 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYL9 fusion protein Ag7600 相同性解析による交差性が予測される生物種
Full Name myosin, light chain 9, regulatory
Calculated molecular weight 20 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC002648
Gene Symbol MYL9
Gene ID (NCBI) 10398
RRIDAB_3083969
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP24844
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Myosin regulatory light polypeptide 9 (MYL9), also known as MLC2, belongs to the myosin regulatory subunits. It plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation at Thr19 and Ser20. Implicated in cytokinesis, receptor capping, and cell locomotion (PMID:11942626, PMID:2526655). Some studies have demonstrated that MYL9 may play important roles in various human cancers. The expression and phosphorylation of MYL9 (Thr19/Ser20) may be increased in human breast (PMID: 22144583) and liver cancers (PMID: 18648664), while decreased in human colon (PMID: 22752057) and bladder cancers (PMID: 21139803). MYL9 was the only gene differentially expressed in the aged versus young injured arteries in the rat smooth muscle cell layers (PMID:22003410).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 MYL9 antibody CL488-15354Download protocol
Standard Protocols
Click here to view our Standard Protocols