Validation Data Gallery

Filter: AllWBIHCIF-PIF/ICCFC (Intra)

WB analysis using 17329-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HT-29 using 17329-1-AP
WB result of Cytokeratin 20 antibody (17329-1-AP; 1:120000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytokeratin 20 transfected HT-29 cells.
WB analysis of HeLa using 17329-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17329-1-AP (Cytokeratin 20 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human colon using 17329-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 17329-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human urothelial carcinoma using 17329-1-AP
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human urothelial carcinoma using 17329-1-AP
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human appendicitis using 17329-1-AP
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human appendicitis using 17329-1-AP
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 17329-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 17329-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 17329-1-AP
Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 17329-1-AP
Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 17329-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 17329-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human urothelial carcinoma using 17329-1-AP
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human urothelial carcinoma using 17329-1-AP
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human colon cancer using 17329-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using Cytokeratin 20 antibody (17329-1-AP) at dilution of 1:200 and CoraLite®647-conjugated F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L) (SA00014-9), Collagen Type III antibody (68320-1-Ig, Clone: 3B1F3, green), CoraLite®594 CD68 antibody (CL594-25747, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
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Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using Cytokeratin 20 antibody (17329-1-AP) at dilution of 1:200 and CoraLite®647-conjugated F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L) (SA00014-9), Collagen Type III antibody (68320-1-Ig, Clone: 3B1F3, green), CoraLite®594 CD68 antibody (CL594-25747, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of Rabbit colon using 17329-1-AP
Immunofluorescent analysis of (4% PFA) fixed Rabbit colon tissue using Cytokeratin 20 antibody (17329-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of Rabbit colon using 17329-1-AP
Immunofluorescent analysis of (4% PFA) fixed Rabbit colon tissue using Cytokeratin 20 antibody (17329-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of Caco-2 using 17329-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using Cytokeratin 20 antibody (17329-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
IF Staining of HeLa using 17329-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HT-29 using 17329-1-AP
1X10^6 HT-29 cells were intracellularly stained with 0.2 ug Anti-Human Cytokeratin 20 (17329-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

KD/KO Validated

Cytokeratin 20 Polyclonal antibody

Cytokeratin 20 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Cat No. 17329-1-AP

Publications(24)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, rabbit

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Synonyms

KRT20, CK 20, CK20, CK-20, Cytokeratin-20

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (24)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	COLO 320 cells, HeLa cells, HT-29 cells
Positive IHC detected in	human colon tissue, human appendicitis tissue, human colon cancer tissue, human stomach cancer tissue, human urothelial carcinoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	Rabbit colon tissue, human colon cancer tissue
Positive IF/ICC detected in	Caco-2 cells, HeLa cells
Positive FC (Intra) detected in	HT-29 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunohistochemistry (IHC)	IHC : 1:800-1:3200
Immunofluorescence (IF)-P	IF-P : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 3 publications below
IHC	See 4 publications below
IF	See 12 publications below

Product Information

17329-1-AP targets Cytokeratin 20 in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

Tested Reactivity	human, mouse, rat, rabbit
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag11308 Product name: Recombinant human KRT20 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 74-424 aa of BC031559 Sequence: MQNLNDRLASYLEKVRTLEQSNSKLEVQIKQWYETNAPRAGRDYSAYYRQIEELRSQIKDAQLQNARCVLQIDNAKLAAEDFRLKYETERGIRLTVEADLQGLNKVFDDLTLHKTDLEIQIEELNKDLALLKKEHQEEVDGLHKHLGNTVNVEVDAAPGLNLGVIMNEMRQKYEVMAQKNLQEAKEQFERQTAVLQQQVTVNTEELKGTEVQLTELRRTSQSLEIELQSHLSMKESLEHTLEETKARYSSQLANLQSLLSSLEAQLMQIRSNMERQNNEYHILLDIKTRLEQEIATYRRLLEGEDVKTTEYQLSTLEERDIKKTRKIKTVVQEVVDGKVVSSEVKEVEENI 相同性解析による交差性が予測される生物種
Full Name	keratin 20
Calculated molecular weight	424 aa, 48 kDa
Observed molecular weight	46 kDa
GenBank accession number	BC031559
Gene Symbol	Cytokeratin 20
Gene ID (NCBI)	54474
RRID	AB_2133592
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P35900
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.

Protocols

Product Specific Protocols
FC protocol for Cytokeratin 20 antibody 17329-1-AP	Download protocol
IF protocol for Cytokeratin 20 antibody 17329-1-AP	Download protocol
IHC protocol for Cytokeratin 20 antibody 17329-1-AP	Download protocol
WB protocol for Cytokeratin 20 antibody 17329-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Mol Cancer DC - SIGNR by influencing the lncRNA HNRNPKP2 upregulates the expression of CXCR4 in gastric cancer liver metastasis. Authors - Yu Zhang View Article
mouse	IF	Cell Res Gut microbiota drives macrophage-dependent self-renewal of intestinal stem cells via niche enteric serotonergic neurons. Authors - Pingping Zhu View Article
mouse,human	IF	Nat Immunol IL-13 secreted by ILC2s promotes the self-renewal of intestinal stem cells through circular RNA circPan3. Authors - Pingping Zhu View Article
mouse	WB	Nat Cell Biol LncGata6 maintains stemness of intestinal stem cells and promotes intestinal tumorigenesis. Authors - Pingping Zhu View Article
		Nat Commun Colorectal cancer co-opts an epidermal wound healing program during metastasis to generate disseminated tumor cells. Authors - Mizuho Sakahara View Article
mouse	IF	Nat Commun Single-cell transcriptomics uncovers EGFR signaling-mediated gastric progenitor cell differentiation in stomach homeostasis Authors - Hitomi Takada View Article

Validation Data Gallery

WB analysis using 17329-1-AP

WB analysis of HT-29 using 17329-1-AP

WB analysis of HeLa using 17329-1-AP

IHC staining of human colon using 17329-1-AP

IHC staining of human colon using 17329-1-AP

IHC staining of human urothelial carcinoma using 17329-1-AP

IHC staining of human urothelial carcinoma using 17329-1-AP

IHC staining of human appendicitis using 17329-1-AP

IHC staining of human appendicitis using 17329-1-AP

IHC staining of human stomach cancer using 17329-1-AP

IHC staining of human stomach cancer using 17329-1-AP

IHC staining of human colon cancer using 17329-1-AP

IHC staining of human colon cancer using 17329-1-AP

IHC staining of human colon using 17329-1-AP

IHC staining of human colon using 17329-1-AP

IHC staining of human urothelial carcinoma using 17329-1-AP

IHC staining of human urothelial carcinoma using 17329-1-AP

IF Staining of human colon cancer using 17329-1-AP

IF Staining of Rabbit colon using 17329-1-AP

IF Staining of Rabbit colon using 17329-1-AP

IF Staining of Caco-2 using 17329-1-AP

IF Staining of HeLa using 17329-1-AP

FC experiment of HT-29 using 17329-1-AP

Cytokeratin 20 Polyclonal antibody

Cytokeratin 20 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

DC - SIGNR by influencing the lncRNA HNRNPKP2 upregulates the expression of CXCR4 in gastric cancer liver metastasis.

Gut microbiota drives macrophage-dependent self-renewal of intestinal stem cells via niche enteric serotonergic neurons.

IL-13 secreted by ILC2s promotes the self-renewal of intestinal stem cells through circular RNA circPan3.

LncGata6 maintains stemness of intestinal stem cells and promotes intestinal tumorigenesis.

Colorectal cancer co-opts an epidermal wound healing program during metastasis to generate disseminated tumor cells.

Single-cell transcriptomics uncovers EGFR signaling-mediated gastric progenitor cell differentiation in stomach homeostasis

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