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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Cytokeratin 20 Polyclonal antibody

Cytokeratin 20 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, rabbit

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-17329

Synonyms

CK 20, CK20, Cytokeratin 20, K20, keratin 20, KRT20, KRT21, Protein IT

Validation Data Gallery

Applications:
  • IF Staining of HT-29 using CL488-17329

    IF Staining of HT-29 using CL488-17329

    Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using CoraLite® Plus 488 Cytokeratin 20 antibody (CL488-17329) at dilution of 1:200.

Tested Applications

Positive IF detected inHT-29 cells
Planning an IHC experiment? We recommend our IHCeasy KRT20 Ready-To-Use IHC Kit. KRT20 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 17329-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-17329 targets Cytokeratin 20 in IF applications and shows reactivity with human, mouse, rat, rabbit samples.

Tested Reactivity human, mouse, rat, rabbit
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 20 fusion protein Ag11308 相同性解析による交差性が予測される生物種
Full Name keratin 20
Calculated molecular weight 424 aa, 48 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC031559
Gene symbol KRT20
Gene ID (NCBI) 54474
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Cytokeratin 20 antibody CL488-17329Download protocol
Standard Protocols
Click here to view our Standard Protocols