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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Importin Beta Polyclonal antibody

Importin Beta Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10077

Synonyms

IMB1, Impnb, Importin 90, Importin subunit beta 1, IPO1, IPOB, karyopherin (importin) beta 1, Karyopherin subunit beta 1, KPNB1, NTF97, Nuclear factor p97, PTAC97

Validation Data Gallery

Applications:
  • FC experiment of Jurkat using CL488-10077

    FC experiment of Jurkat using CL488-10077

    1X10^6 Jurkat cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Importin Beta (CL488-10077) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy KPNB1 Ready-To-Use IHC Kit. KPNB1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10077-1-AP

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-10077 targets Importin Beta in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Importin Beta fusion protein Ag0122 相同性解析による交差性が予測される生物種
Full Name karyopherin (importin) beta 1
Calculated molecular weight 97 kDa
Observed molecular weight 90-97 kDa
GenBank accession numberBC003572
Gene symbol KPNB1
Gene ID (NCBI) 3837
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Importin beta1, also known karyopherin beta or KPNB1, is a nuclear transport receptor that plays a key role in the nuclear import process. Importin beta1 and importin alpha work in concert to transport cargo into the nucleus, functioning as an essential component of the classical nuclear localization signal (NLS) pathway. Elevated expression of importin beta1 has been found in cervical tumors. (22125623)

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 Importin Beta antibody CL488-10077Download protocol
Standard Protocols
Click here to view our Standard Protocols