Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 10077-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10077-1-AP (Importin beta antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 10077-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10077-1-AP (Importin Beta antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 10077-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10077-1-AP (Importin Beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 10077-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10077-1-AP (Importin Beta antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 10077-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10077-1-AP (Importin Beta antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10077-1-AP
WB result of Importin Beta antibody (10077-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Importin Beta transfected HeLa cells.
WB analysis of rat brain using 10077-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 10077-1-AP (Importin beta antibody at dilution of 1:600 incubated at 4 degree celsius over night.
WB analysis of mouse brain using 10077-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10077-1-AP (Importin beta antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of A2780 using 10077-1-AP
IP result of anti-Importin Beta (IP:10077-1-AP, 4ug; Detection:10077-1-AP 1:5000) with A2780 cells lysate 760 ug.
IHC staining of mouse brain using 10077-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10077-1-AP (Importin Beta antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 10077-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10077-1-AP (Importin Beta antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10077-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10077-1-AP (Importin Beta antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 10077-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Importin Beta antibody (10077-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HeLa using 10077-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Importin Beta antibody (10077-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HeLa using 10077-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Importin Beta antibody (10077-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of Jurkat using 10077-1-AP
1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human Importin Beta (10077-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

Importin Beta Polyclonal antibody

Importin Beta Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 10077-1-AP

Publications(28)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

KPNB1, IMB1, Impnb, Importin 90, Importin subunit beta 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (28)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A2780 cells, HeLa cells, mouse brain tissue, rat brain tissue, HT-1080 cells, NIH/3T3 cells, C6 cells, PC-12 cells
Positive IP detected in	A2780 cells
Positive IHC detected in	human colon cancer tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	Jurkat cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 25 publications below
IHC	See 3 publications below
IF	See 3 publications below
IP	See 4 publications below
CoIP	See 3 publications below

Product Information

10077-1-AP targets Importin Beta in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0122 Product name: Recombinant human KPNB1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 409-612 aa of BC003572 Sequence: AMPTLIELMKDPSVVVRDTAAWTVGRICELLPEAAINDVYLAPLLQCLIEGLSAEPRVASNVCWAFSSLAEAAYEAADVADDQEEPATYCLSSSFELIVQKLLETTDRPDGHQNNLRSSAYESLMEIVKNSAKDCYPAVQKTTLVIMERLQQVLQMESHIQSTSDRIQFNDLQSLLCATLQNVLRKVQHQDALQISDVVMASLL 相同性解析による交差性が予測される生物種
Full Name	karyopherin (importin) beta 1
Calculated molecular weight	97 kDa
Observed molecular weight	90-97 kDa
GenBank accession number	BC003572
Gene Symbol	KPNB1
Gene ID (NCBI)	3837
RRID	AB_2133977
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q14974
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Importin beta1, also known karyopherin beta or KPNB1, is a nuclear transport receptor that plays a key role in the nuclear import process. Importin beta1 and importin alpha work in concert to transport cargo into the nucleus, functioning as an essential component of the classical nuclear localization signal (NLS) pathway. Elevated expression of importin beta1 has been found in cervical tumors. (PMID: 22125623)

Protocols

Product Specific Protocols
FC protocol for Importin Beta antibody 10077-1-AP	Download protocol
IF protocol for Importin Beta antibody 10077-1-AP	Download protocol
IHC protocol for Importin Beta antibody 10077-1-AP	Download protocol
IP protocol for Importin Beta antibody 10077-1-AP	Download protocol
WB protocol for Importin Beta antibody 10077-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Cancer CREB3L1 promotes tumor growth and metastasis of anaplastic thyroid carcinoma by remodeling the tumor microenvironment Authors - Zongfu Pan View Article
human	WB,IP	Cell Res Neddylation of PTEN regulates its nuclear import and promotes tumor development. Authors - Ping Xie View Article
human	WB	Nat Cancer Phase separation of phospho-HDAC6 drives aberrant chromatin architecture in triple-negative breast cancer Authors - Bing Lu View Article
human	WB	Autophagy TMX2 potentiates cell viability of hepatocellular carcinoma by promoting autophagy and mitophagy. Authors - Weiyu Zhang View Article
mouse	WB,IHC	Autophagy Ablation of endothelial Atg7 inhibits ischemia-induced angiogenesis by upregulating Stat1 that suppresses Hif1a expression Authors - Hongmin Yao View Article
human	WB	Mol Cell EGFR promotes ALKBH5 nuclear retention to attenuate N6-methyladenosine and protect against ferroptosis in glioblastoma Authors - Deguan Lv View Article

Validation Data Gallery

WB analysis using 10077-1-AP

WB analysis using 10077-1-AP

WB analysis using 10077-1-AP

WB analysis using 10077-1-AP

WB analysis using 10077-1-AP

WB analysis of HeLa using 10077-1-AP

WB analysis of rat brain using 10077-1-AP

WB analysis of mouse brain using 10077-1-AP

IP experiment of A2780 using 10077-1-AP

IHC staining of mouse brain using 10077-1-AP

IHC staining of mouse brain using 10077-1-AP

IHC staining of human colon cancer using 10077-1-AP

IF Staining of HeLa using 10077-1-AP

IF Staining of HeLa using 10077-1-AP

IF Staining of HeLa using 10077-1-AP

FC experiment of Jurkat using 10077-1-AP

Importin Beta Polyclonal antibody

Importin Beta Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

CREB3L1 promotes tumor growth and metastasis of anaplastic thyroid carcinoma by remodeling the tumor microenvironment

Neddylation of PTEN regulates its nuclear import and promotes tumor development.

Phase separation of phospho-HDAC6 drives aberrant chromatin architecture in triple-negative breast cancer

TMX2 potentiates cell viability of hepatocellular carcinoma by promoting autophagy and mitophagy.

Ablation of endothelial Atg7 inhibits ischemia-induced angiogenesis by upregulating Stat1 that suppresses Hif1a expression

EGFR promotes ALKBH5 nuclear retention to attenuate N6-methyladenosine and protect against ferroptosis in glioblastoma

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