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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated IgM Monoclonal antibody

human IgM Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2D10B10

Cat no : CL594-66484

Synonyms

DKFZp686I15196, DKFZp686I15212, FLJ00385, human IgM, IGHM, IgM, MU, VH

Validation Data Gallery

Applications:
  • IF Staining of human tonsillitis using CL594-66484

    IF Staining of human tonsillitis using CL594-66484

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 human IgM antibody (CL594-66484, Clone: 2D10B10 ) at dilution of 1:100, CoraLite® Plus 488 CD3 antibody (CL488-60181, Clone: 3F3A1, green), CoraLite® Plus 647 human IgD antibody (CL647-16489, Magenta).

  • IF Staining of human tonsillitis using CL594-66484

    IF Staining of human tonsillitis using CL594-66484

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 human IgM antibody (CL594-66484, Clone: 2D10B10 ) at dilution of 1:100, CoraLite® Plus 488 CD3 antibody (CL488-60181, Clone: 3F3A1, green), CoraLite® Plus 647 human IgD antibody (CL647-16489, Magenta).

  • IF Staining of human appendicitis using CL594-66484

    IF Staining of human appendicitis using CL594-66484

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CoraLite®594 human IgM antibody (CL594-66484, Clone: 2D10B10 ) at dilution of 1:200.

  • IF Staining of human tonsillitis using CL594-66484

    IF Staining of human tonsillitis using CL594-66484

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594-conjugated human IgM antibody (CL594-66484, Clone: 2D10B10 ) at dilution of 1:100.

  • IF Staining of human tonsillitis using CL594-66484

    IF Staining of human tonsillitis using CL594-66484

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594-conjugated human IgM antibody (CL594-66484, Clone: 2D10B10 ) at dilution of 1:100.

Tested Applications

Positive IF detected inhuman tonsillitis tissue, human appendicitis tissue
Planning an IHC experiment? We recommend our IHCeasy human IgM Ready-To-Use IHC Kit. human IgM primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66484-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-66484 targets human IgM in IF applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen human IgM fusion protein Ag1459 相同性解析による交差性が予測される生物種
Full Name immunoglobulin heavy constant mu
Calculated molecular weight 69 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC009851
Gene symbol IGHM
Gene ID (NCBI) 3507
RRIDAB_2883590
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL594 human IgM antibody CL594-66484Download protocol
Standard Protocols
Click here to view our Standard Protocols