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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite® Plus 647-conjugated human IgD Polyclonal (CL647-16489)

    IF Staining of human tonsillitis using CL647-16489

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®®647 human IgD antibody (CL647-16489) at dilution of 1:200.

CoraLite® Plus 647-conjugated IgD Polyclonal antibody

human IgD Polyclonal Antibody for IF-P
Cat No. CL647-16489

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF-P

Synonyms

FLJ00382, FLJ46727, IgD, IGHD

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 647
Size/Concentration: 

-/ -

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Tested Applications

Positive IF-P detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-16489 targets human IgD in IF-P applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9614

Product name: Recombinant human IGHD protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 202-548 aa of BC021276

Sequence: RRDSYYMTSSQLSTPLQQWRQGEYKCVVQHTASKSKKEIFRWPESPKAQASSVPTAQPQAEGSLAKATTAPATTRNTGRGGEEKKKEKEKEEQEERETKTPECPSHTQPLGVYLLTPAVQDLWLRDKATFTCFVVGSDLKDAHLTWEVAGKVPTGGVEEGLLERHSNGSQSQHSRLTLPRSLWNAGTSVTCTLNHPSLPPQRLMALREPAAQAPVKLSLNLLASSDPPEAASWLLCEVSGFSPPNILLMWLEDQREVNTSGFAPARPPPQPGSTTFWAWSVLRVPAPPSPQPATYTCVVSHEDSRTLLNASRSLEVSYLAMTPLIPQSKDENSDDYTTFDDVGSLWT

相同性解析による交差性が予測される生物種
Full Name immunoglobulin heavy constant delta
Calculated molecular weight 573 aa, 63 kDa
GenBank accession numberBC021276
Gene Symbol IGHD
Gene ID (NCBI) 3495
RRIDAB_2920231
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form
FormLiquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IgD is the major antigen receptor isotype on the surface of most peripheral B-cells, where it is coexpressed with IgM. The membrane-bound IgD (mIgD) induces the phosphorylation of CD79A and CD79B by the Src family of protein tyrosine kinases. This antibody recognize the heavy chain (~60 kDa) of IgD.

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 human IgD antibody CL647-16489Download protocol
Standard Protocols
Click here to view our Standard Protocols