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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated HSP47 Monoclonal antibody (CL594-67863)

    IF Staining of HepG2 using CL594-67863

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 HSP47 antibody (CL594-67863, Clone: 2A8F6 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated HSP47 Monoclonal antibody (CL594-67863)

    IF Staining of HeLa using CL594-67863

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 HSP47 antibody (CL594-67863, Clone: 2A8F6 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite®594-conjugated HSP47 Monoclonal antibody (CL594-67863)

    FC experiment of HepG2 using CL594-67863

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human HSP47 (CL594-67863, Clone:2A8F6) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated HSP47/SERPINH1 Monoclonal antibody

HSP47/SERPINH1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-67863
Clone No.2A8F6

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

HSP47, SERPINH1, 2A8F6, 47 kDa heat shock protein, Arsenic-transactivated protein 3

Formulation:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells, HepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67863 targets HSP47/SERPINH1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag30999

Product name: Recombinant human SERPINH1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-418 aa of BC014623

Sequence: MRSLLLLSAFCLLEAALAAEVKKPAAAAAPGTAEKLSPKAATLAERSAGLAFSLYQAMAKDQAVENILVSPVVVASSLGLVSLGGKATTASQAKAVLSAEQLRDEEVHAGLGELLRSLSNSTARNVTWKLGSRLYGPSSVSFADDFVRSSKQHYNCEHSKINFRDKRSALQSINEWAAQTTDGKLPEVTKDVERTDGALLVNAMFFKPHWDEKFHHKMVDNRGFMVTRSYTVGVMMMHRTGLYNYYDDEKEKLQIVEMPLAHKLSSLIILMPHHVEPLERLEKLLTKEQLKIWMGKMQKKAVAISLPKGVVEVTHDLQKHLAGLGLTEAIDKNKADLSRMSGKKDLYLASVFHATAFELDTDGNPFDQDIYGREELRSPKLFYADHPFIFLVRDTQSGSLLFIGRLVRPKGDKMRDEL

相同性解析による交差性が予測される生物種
Full Name serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
Calculated molecular weight 46 kDa
Observed molecular weight46 kDa
GenBank accession numberBC014623
Gene Symbol HSP47/SERPINH1
Gene ID (NCBI) 871
RRIDAB_2923928
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP50454
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL594 HSP47/SERPINH1 antibody CL594-67863Download protocol
Standard Protocols
Click here to view our Standard Protocols
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