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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated HIBCH Monoclonal ant (CL488-68157)

    IF Staining of HepG2 using CL488-68157

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 HIBCH antibody (CL488-68157, Clone: 1B7B8 ) at dilution of 1:100.

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  • KD/KO Validated

CoraLite® Plus 488-conjugated HIBCH Monoclonal antibody

HIBCH Monoclonal Antibody for IF/ICC
Cat No. CL488-68157
Clone No.1B7B8

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

IF/ICC

Synonyms

HIBYL COA H, HIB-CoA hydrolase, HIB CoA hydrolase, EC:3.1.2.4, 3-hydroxyisobutyryl-coenzyme A hydrolase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68157 targets HIBCH in IF/ICC applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen HIBCH fusion protein Ag6473 相同性解析による交差性が予測される生物種
Full Name 3-hydroxyisobutyryl-Coenzyme A hydrolase
Calculated molecular weight 43 kDa
Observed molecular weight38 kDa
GenBank accession numberBC067822
Gene Symbol HIBCH
Gene ID (NCBI) 26275
RRIDAB_3084423
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6NVY1
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HIBCH belongs to the enoyl-CoA hydratase/isomerase family. HIBCH has two isoforms with MW 43 kDa and 38 kDa. It is highly expressed in the liver and kidney, also detected in the heart, muscle and brain (at protein level), but not detected in the lung. Hydrolyzes 3-hydroxyisobutyryl-CoA (HIBYL-CoA), a saline catabolite. Has high activity toward isobutyryl-CoA. Could be an isobutyryl-CoA dehydrogenase that functions in valine catabolism. Also hydrolyzes 3-hydroxypropanoyl-CoA (PMID: 8824301).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 HIBCH antibody CL488-68157Download protocol
Standard Protocols
Click here to view our Standard Protocols