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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using HIBCH Monoclonal antibody (68157-1-Ig)

    WB analysis of HepG2 using 68157-1-Ig

    WB result of HIBCH antibody (68157-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HIBCH transfected HepG2 cells.

  • Western Blot (WB) analysis of various lysates using HIBCH Monoclonal antibody (68157-1-Ig)

    WB analysis using 68157-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68157-1-Ig (HIBCH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HIBCH Monoclonal antibody (68157-1-Ig)

    IF Staining of HepG2 using 68157-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HIBCH antibody (68157-1-Ig, Clone: 1B7B8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

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  • KD/KO Validated

HIBCH Monoclonal antibody

HIBCH Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68157-1-Ig
Clone No.1B7B8

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, ELISA

Synonyms

HIBYL COA H, HIB-CoA hydrolase, HIB CoA hydrolase, EC:3.1.2.4, 3-hydroxyisobutyryl-coenzyme A hydrolase

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells, K-562 cells, pig heart tissue, rabbit heart tissue, rat heart tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68157-1-Ig targets HIBCH in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen HIBCH fusion protein Ag6473 相同性解析による交差性が予測される生物種
Full Name 3-hydroxyisobutyryl-Coenzyme A hydrolase
Calculated molecular weight 43 kDa
Observed molecular weight38 kDa
GenBank accession numberBC067822
Gene Symbol HIBCH
Gene ID (NCBI) 26275
RRIDAB_2935261
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6NVY1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HIBCH belongs to the enoyl-CoA hydratase/isomerase family. HIBCH has two isoforms with MW 43 kDa and 38 kDa. It is highly expressed in the liver and kidney, also detected in the heart, muscle and brain (at protein level), but not detected in the lung. Hydrolyzes 3-hydroxyisobutyryl-CoA (HIBYL-CoA), a saline catabolite. Has high activity toward isobutyryl-CoA. Could be an isobutyryl-CoA dehydrogenase that functions in valine catabolism. Also hydrolyzes 3-hydroxypropanoyl-CoA (PMID: 8824301).

Protocols

Product Specific Protocols
WB protocol for HIBCH antibody 68157-1-IgDownload protocol
IF protocol for HIBCH antibody 68157-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols