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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Glycophorin A Polyclonal antibody

Glycophorin A Polyclonal Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-15874

Synonyms

CD235a, Glycophorin A, GPA, GPErik, GpMiIII, GPSAT, GYPA, HGpMiIII, HGpMiV, HGpMiX, HGpMiXI, HGpSta(C), MN, MN sialoglycoprotein, MNS, PAS 2, Sialoglycoprotein alpha

Validation Data Gallery

Applications:
  • FC experiment of K-562 using CL488-15874

    FC experiment of K-562 using CL488-15874

    1x10^6 K-562 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Glycophorin A (CL488-15874) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were not fixed.

Tested Applications

Positive FC detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy Glycophorin A Ready-To-Use IHC Kit. Glycophorin A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 15874-1-AP

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-15874 targets Glycophorin A in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Glycophorin A fusion protein Ag8635 相同性解析による交差性が予測される生物種
Full Name glycophorin A (MNS blood group)
Calculated molecular weight 150 aa, 16 kDa
Observed molecular weight 36-38 kDa
GenBank accession numberBC005319
Gene symbol GYPA
Gene ID (NCBI) 2993
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glycophorin A (GYPA) is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 Glycophorin A antibody CL488-15874Download protocol
Standard Protocols
Click here to view our Standard Protocols