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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Glycophorin A Polyclonal antibody

Glycophorin A Polyclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15874-1-AP

Synonyms

CD235a, Glycophorin A, GPA, GPErik, GpMiIII, GPSAT, GYPA, HGpMiIII, HGpMiV, HGpMiX, HGpMiXI, HGpSta(C), MN, MN sialoglycoprotein, MNS, PAS 2, Sialoglycoprotein alpha

Validation Data Gallery

Applications:
  • WB analysis of K-562 using 15874-1-AP

    WB analysis of K-562 using 15874-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 15874-1-AP (Glycophorin A antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 15874-1-AP

    WB analysis of K-562 using 15874-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 15874-1-AP (Glycophorin A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human spleen using 15874-1-AP

    IHC staining of human spleen using 15874-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 15874-1-AP (Glycophorin A antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with Glycophorin A antibody included
  • IHC staining of human spleen using 15874-1-AP

    IHC staining of human spleen using 15874-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 15874-1-AP (Glycophorin A antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with Glycophorin A antibody included
  • IHC staining of human colon cancer using 15874-1-AP

    IHC staining of human colon cancer using 15874-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15874-1-AP (Glycophorin A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Glycophorin A antibody included
  • IHC staining of human colon cancer using 15874-1-AP

    IHC staining of human colon cancer using 15874-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15874-1-AP (Glycophorin A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Glycophorin A antibody included
  • IHC staining of human lung using 15874-1-AP

    IHC staining of human lung using 15874-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15874-1-AP (Glycophorin A antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Glycophorin A antibody included
  • IHC staining of human lung using 15874-1-AP

    IHC staining of human lung using 15874-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15874-1-AP (Glycophorin A antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Glycophorin A antibody included
  • IF Staining of human placenta using 15874-1-AP

    IF Staining of human placenta using 15874-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using Glycophorin A antibody (15874-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of human placenta using 15874-1-AP

    IF Staining of human placenta using 15874-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using Glycophorin A antibody (15874-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of K-562 using 15874-1-AP

    FC experiment of K-562 using 15874-1-AP

    1x10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human Glycophorin A (15874-1-AP)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP). Cells were not fixed.

Tested Applications

Positive WB detected inK-562 cells
Positive IHC detected inhuman spleen tissue, human lung tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman placenta tissue
Positive FC detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy Glycophorin A Ready-To-Use IHC Kit. Glycophorin A primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:300-1:1200
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

15874-1-AP targets Glycophorin A in WB, IF, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Glycophorin A fusion protein Ag8635 相同性解析による交差性が予測される生物種
Full Name glycophorin A (MNS blood group)
Calculated molecular weight 150 aa, 16 kDa
Observed molecular weight 36-38 kDa
GenBank accession numberBC005319
Gene symbol GYPA
Gene ID (NCBI) 2993
RRIDAB_2116233
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glycophorin A (GYPA) is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)

Protocols

Product Specific Protocols
WB protocol for Glycophorin A antibody 15874-1-APDownload protocol
IHC protocol for Glycophorin A antibody 15874-1-APDownload protocol
IF protocol for Glycophorin A antibody 15874-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols