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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated GNAS Monoclonal antibody

GNAS Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1A9C10

Cat no : CL594-66253

Synonyms

AHO, C20orf45, dJ309F20.1.1, dJ806M20.3.3, GNAS, GNAS complex locus, GNAS1, GPSA, GSA, GSP, NESP, NESP55, PHP1A, PHP1B, POH, Protein ALEX

Validation Data Gallery

Applications:
  • IF Staining of human colon cancer using CL594-66253

    IF Staining of human colon cancer using CL594-66253

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 GNAS antibody (CL594-66253, Clone: 1A9C10 ) at dilution of 1:200.

  • IF Staining of human colon cancer using CL594-66253

    IF Staining of human colon cancer using CL594-66253

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 GNAS antibody (CL594-66253, Clone: 1A9C10 ) at dilution of 1:200.

  • IF Staining of human colon cancer using CL594-66253

    IF Staining of human colon cancer using CL594-66253

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 GNAS antibody (CL594-66253, Clone: 1A9C10 ) at dilution of 1:200.

  • IF Staining of MCF-7 using CL594-66253

    IF Staining of MCF-7 using CL594-66253

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite®594 GNAS antibody (CL594-66253, Clone: 1A9C10 ) at dilution of 1:200.

  • FC experiment of MCF-7 using CL594-66253

    FC experiment of MCF-7 using CL594-66253

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human GNAS (CL594-66253, Clone:1A9C10) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inhuman colon cancer tissue, MCF-7 cells
Positive FC detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 66253-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL594-66253 targets GNAS in IF, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen GNAS fusion protein Ag20530 相同性解析による交差性が予測される生物種
Full Name GNAS complex locus
Calculated molecular weight 45 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC002722
Gene symbol GNAS
Gene ID (NCBI) 2778
RRIDAB_2919971
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 (GNAS1) is the ubiquitously expressed heterotrimeric G protein that couples receptors to the effector enzyme adenylyl cyclase and is required for receptor-stimulated intracellular cAMP generation. Mutations of Gs(alpha) residues involved in the GTPase reaction that lead to constitutive activation are present in endocrine tumors, fibrous dysplasia of bone, and McCune-Albright syndrome. The molecular weight of Gs(alpha) protein is about 46 kDa.

Protocols

Product Specific Protocols
IF protocol for CL594 GNAS antibody CL594-66253Download protocol
FC protocol for CL594 GNAS antibody CL594-66253Download protocol
Standard Protocols
Click here to view our Standard Protocols